Broad diversity of viable bacteria in 'sterile' (0.2 microm) filtered water

Research in Microbiology
Martin W Hahn

Abstract

Filtration of liquids through 0.2-microm filters is a common and often-used method for the removal of microorganisms from heat-sensitive solutions. Such 0.2 microm filtrations are frequently referred to as 'sterile filtration', reflecting the general belief that all living organisms are excluded from the filtrate. However, it has been demonstrated that a few bacterial species can pass through 0.2-microm filters. Despite these observations, it is generally assumed that most bacteria are unable to pass through these filters. In contrast to this assumption, a wide diversity of 19 bacterial taxa was isolated by the acclimatization method from 0.2 microm-filtrated freshwater samples. All isolates were able to grow on substrate-rich agar plates. The size of the isolated strains ranged from <1 to >10 microm in cell length. The obtained isolates belong to the Bacteroidetes, Alphaproteobacteria, Betaproteobacteria, Actinobacteria and Spirochaetes. Previous studies which did not use the acclimatization method reported that only two out of the 19 taxa passed through 0.2- or 0.45-microm filters. The majority of the obtained isolates were more closely related to uncultured than to previously cultured bacteria. It is assumed that the obtaine...Continue Reading

References

Jan 1, 1977·Annual Review of Microbiology·R C Johnson
Sep 30, 1975·Archives of Microbiology·J A Breznak, E Canale-Parola
Sep 1, 1968·Canadian Journal of Microbiology·J L BraunW F McCulloch
Nov 24, 1999·Home Healthcare Nurse·P L Davis, E A Madigan
Mar 7, 2003·Applied and Environmental Microbiology·Martin W HahnPeter Stadler
May 12, 2004·Journal of Microbiological Methods·Martin W HahnMatthias Pöckl

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Citations

Dec 17, 2014·Mikrobiologiia·A V FedotovaS N Dedysh
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jaan MännikCees Dekker
Oct 1, 2013·Applied and Environmental Microbiology·Maria C PortilloNoah Fierer
Aug 10, 2012·BMC Research Notes·Nuno F AzevedoMaria J Vieira
Apr 3, 2007·Extremophiles : Life Under Extreme Conditions·Takeshi NaganumaHiroyuki Kimura
Feb 1, 2011·Marine Biotechnology·Ryosuke NakaiTakeshi Naganuma
May 4, 2007·Journal of Industrial Microbiology & Biotechnology·Daniela Santos PontesAndréa Maria Amaral Nascimento
Oct 7, 2015·Frontiers in Microbiology·Mária DžunkováAndrés Moya
May 4, 2010·International Journal of Systematic and Evolutionary Microbiology·Martin W HahnCathrin Spröer
Mar 29, 2011·International Journal of Systematic and Evolutionary Microbiology·Martin W HahnCatrin Spröer
Sep 12, 2009·FEMS Microbiology Reviews·Vincent ThomasJean-Yves Maillard
Jun 26, 2018·International Journal of Systematic and Evolutionary Microbiology·Martin W HahnElke Lang
Dec 2, 2008·Journal of Applied Microbiology·F S Silbaq
Jul 22, 2015·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Heather Moulton-MeissnerMatthew Arduino
Nov 7, 2019·Critical Reviews in Microbiology·Pompeyo FerroCélia M Manaia
Jun 30, 2007·Microbiology·Marius VitalThomas Egli
Sep 10, 2020·Proceedings. Biological Sciences·Andrew M RameyDavid E Stallknecht
Jul 15, 2017·Frontiers in Microbiology·Marta MaciejewskaSébastien Rigali
Nov 19, 2011·FEMS Microbiology Ecology·Rebekah R HeltonK Eric Wommack
Aug 2, 2018·Frontiers in Microbiology·Flavia FlavianiEdward P Rybicki
Nov 22, 2020·Rapid Communications in Mass Spectrometry : RCM·Jean-François Hélie, Claude Hillaire-Marcel
Dec 3, 2020·FEMS Microbiology Ecology·Lydia-Ann J GhuneimDavid L Jones
Sep 20, 2008·Environmental Science & Technology·Yingying WangThomas Egli

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