Broad, high-magnitude and multifunctional CD4+ and CD8+ T-cell responses elicited by a DNA and modified vaccinia Ankara vaccine containing human immunodeficiency virus type 1 subtype C genes in baboons

The Journal of General Virology
Wendy A BurgersAnna-Lise Williamson

Abstract

Candidate human immunodeficiency virus (HIV) vaccine regimens based on DNA boosted with recombinant modified vaccinia Ankara (MVA) have been in development for some time, and there is evidence for improved immunogenicity of newly developed constructs. This study describes immune responses to candidate DNA and MVA vaccines expressing multiple genes (gag, RT, tat, nef and env) from HIV-1 subtype C in chacma baboons (Papio ursinus). The vaccine regimen induced (i) strong T-cell responses, with a median of 4103 spot forming units per 10(6) peripheral blood mononuclear cells by gamma interferon (IFN-gamma) ELISPOT, (ii) broad T-cell responses targeting all five vaccine-expressed genes, with a median of 12 peptides targeted per animal and without any single protein dominating the response, (iii) balanced CD4(+) and CD8(+) responses, which produced both IFN-gamma and interleukin (IL)-2, including IL-2-only responses not detected by the ELISPOT assay, (iv) vaccine memory, which persisted 1 year after immunization and could be boosted further, despite strong anti-vector responses, and (v) mucosal T-cell responses in iliac and mesenteric lymph nodes in two animals tested. The majority of peptide responses mapped contained epitopes previo...Continue Reading

References

May 16, 1998·Tissue Antigens·L K GaurJ Anderson
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D A SidebottomW H Hildebrand
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zdenek HelGenoveffa Franchini
Mar 18, 2003·AIDS Research and Human Retroviruses·Carolyn WilliamsonRonald Swanstrom
Jun 29, 2004·Journal of Virology·Norman L LetvinGary J Nabel
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heather D Hickman-MillerWilliam H Hildebrand
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zdenek HelGenoveffa Franchini
Jun 1, 2006·The Journal of Experimental Medicine·Joseph J MattapallilMario Roederer
Jul 11, 2006·Expert Review of Vaccines·Barton F Haynes, David C Montefiori
Jul 14, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ann DuerrLawrence Corey
Jul 29, 2006·Journal of Medical Primatology·Joseph J MattapallilMario Roederer
Nov 17, 2006·The Journal of Infectious Diseases·Barney S GrahamUNKNOWN Vaccine Research Center 004 Study Team

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Citations

Aug 4, 2010·Immunology and Cell Biology·Paweena RattanasenaAndreas Suhrbier
Nov 10, 2010·AIDS·Patricia E Fast, Pontiano Kaleebu
Aug 1, 2012·PloS One·Lech IgnatowiczAndrzej Pawlowski
Dec 10, 2015·Expert Review of Vaccines·Anna-Lise Williamson, Edward P Rybicki
Aug 4, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jie Zhong, Rajiv Khanna
Nov 3, 2009·Vaccine·Kristopher KolibabSheldon L Morris
Nov 11, 2015·Human Vaccines & Immunotherapeutics·Edward Rybicki
Oct 9, 2009·International Reviews of Immunology·Antonella CaputoBarbara Ensoli

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