PMID: 7012838Dec 1, 1980Paper

Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti

Proceedings of the National Academy of Sciences of the United States of America
G DittaD R Helinski

Abstract

A broad host range cloning vehicle that can be mobilized at high frequency into Gram-negative bacteria has been constructed from the naturally occurring antibiotic resistance plasmid RK2. The vehicle is 20 kilobase pairs in size, encodes tetracycline resistance, and contains two single restriction enzyme sites suitable for cloning. Mobilization is effected by a helper plasmid consisting of the RK2 transfer genes linked to a ColE1 replicon. By use of this plasmid vehicle, a gene bank of the DNA from a wild-type strain of Rhizobium meliloti has been constructed and established in Escherichia coli. One of the hybrid plasmids in the bank contains a DNA insert of approximately 26 kilobase pairs which has homology to the nitrogenase structural gene region of Klebsiella pneumoniae.

References

Sep 6, 1977·Biochimica Et Biophysica Acta·R J Meyer, D R Helinski
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G E RiedelF C Cannon
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Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness
May 1, 1972·Journal of General Microbiology·N Datta, R W Hedges
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G B Ruvkun, F M Ausubel

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Citations

Sep 6, 2000·Biotechnology and Bioengineering·R Montalvo-RodriguezP Blum
Apr 15, 1993·Biotechnology and Bioengineering·N C Pagratis, H R Revel
Jan 1, 1988·Plant Molecular Biology·C J WoolstonP M Mullineaux
Jan 1, 1989·Plant Molecular Biology·H P SpainkB E Lugtenberg
Sep 1, 1988·Plant Molecular Biology·T J HigginsS Craig
Jan 1, 1987·Plant Molecular Biology·H P SpainkB J Lugtenberg
Nov 1, 1987·Plant Molecular Biology·T D McKnightR B Simpson
Nov 1, 1987·Plant Molecular Biology·D W White, D Greenwood
Nov 1, 1986·Plant Molecular Biology·M A DjordjevicB G Rolfe
Jul 1, 1989·Plant Molecular Biology·B H TaylorW J Peacock
May 1, 1988·Plant Molecular Biology·S J SandlerP Dunsmuir
Dec 1, 1992·Plant Molecular Biology·X Q LiT K Hodges
Sep 1, 1986·Plant Molecular Biology·A J Matzke, M A Matzke

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