Broad spectral excitation of opsin for enhanced stimulation of cells

Optics Letters
Sarmishtha SatpathyS K Mohanty

Abstract

Optical stimulation of cells expressing light-sensitive proteins (opsins) has allowed targeted activation with cellular specificity. However, since narrow-band light has been used for excitation of these optogenetic probes, only active stimulation strategies are being attempted for clinical applications such as restoration of vision. Here, we report use of broad spectral excitation (white light) for optogenetic stimulation of opsin-sensitized cells. We found that ReaChR is optimally excited with white light offering significantly higher photocurrents compared to spectrally filtered narrow-band light stimulation. Our findings open up the possibility of passive stimulation strategy by use of natural sunlight for retinal stimulation, which could have benefits for ambient light stimulated vision restoration.

References

Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Nov 23, 2006·Lancet·Dyonne T HartongThaddeus P Dryja
Apr 25, 2007·Ophthalmic Genetics·Eedy MezerAlex V Levin
Sep 13, 2012·IEEE Transactions on Bio-medical Engineering·Hung CaoJ-C Chiao
Feb 28, 2013·Nature Communications·Inna Reutsky-GefenShy Shoham
Jun 1, 2013·Optics Letters·K DhakalS K Mohanty
Aug 8, 2014·Visual Neuroscience·John Martin BarrettPatrick Degenaar

Citations

Nov 30, 2016·Biomedical Optics Express·Jian-Hong ChenLon A Wang
May 14, 2020·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Silvia GhirgaMarco Leonetti

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