Broad-spectrum antimicrobial activity of human intestinal defensin 5.

Infection and Immunity
E M PorterT Ganz

Abstract

Defensins are antibiotic peptides expressed in human and animal myeloid and epithelial cells. Due to the limited availability of natural peptides, the properties of human epithelial defensins have not been studied. We assayed the microbicidal activity of recombinant human intestinal defensin 5 (rHD-5) in the presence of salt (O to 150 mM NaCl) with varied pH (pH 5.5 to pH 8.5) and trypsin (25 and 250 microg/ml). rHD-5 exhibits microbicidal activity against Listeria monocytogenes, Escherichia coli, and Candida albicans. In contrast to cryptdins, the mouse intestinal defensins, rHD-5 is active against both mouse-virulent wild-type Salmonella typhimurium and its isogenic, mouse-avirulent phoP mutant. In the presence of salt, rHD-5 activity was reduced, and at 100 mM NaCl, activity against S. typhimurium was abolished. However, at all salt concentrations tested, rHD-5 remained bactericidal to L. monocytogenes. Activity against L. monocytogenes was not pH dependent but was diminished at pH 5.5 against wild-type S. typhimurium. This acid-induced resistance may have been mediated by the virulence gene regulator phoP, since the phoP mutant was equally sensitive at pH 5.5 and pH 7.4. In the presence of trypsin, rHD-5 was partially cleav...Continue Reading

References

Dec 1, 1992·International Journal of Peptide and Protein Research·A G RaoJ Duvick
Sep 1, 1992·Cornea·I R SchwabC J Murphy
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M Alpuche ArandaS I Miller
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E A GroismanF Heffron
Sep 1, 1992·Infection and Immunity·P B EisenhauerR I Lehrer
Aug 1, 1992·The Journal of Cell Biology·M E SelstedA J Ouellette
Feb 1, 1992·Antimicrobial Agents and Chemotherapy·R BessalleM Fridkin
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B L KaganR I Lehrer
Feb 15, 1990·Biochemical Pharmacology·B A BerkowitzM A Zasloff
Sep 1, 1991·Molecular Microbiology·S I Miller
Mar 21, 1991·Journal of Immunological Methods·R I LehrerP Eisenhauer
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G DiamondC L Bevins
Jan 1, 1990·Annual Review of Biochemistry·C L Bevins, M Zasloff
Aug 1, 1990·Journal of Pediatric Gastroenterology and Nutrition·J FallingborgS N Rasmussen
Jan 1, 1986·Scandinavian Journal of Gastroenterology. Supplement·M BoheK Ohlsson
Jul 1, 1989·Infection and Immunity·P B EisenhauerR I Lehrer
Aug 1, 1989·The Journal of Clinical Investigation·R I LehrerM E Selsted
Oct 1, 1985·The Journal of Clinical Investigation·T GanzR I Lehrer
Dec 1, 1986·Journal of Virology·K A DaherR I Lehrer
Jul 1, 1985·Infection and Immunity·M E SelstedR I Lehrer
Apr 8, 1972·British Medical Journal·B W WatsonG E Sladen
Aug 1, 1995·Infection and Immunity·M ShimodaO Kohashi
Jul 17, 1995·FEBS Letters·K W BenschW G Forssmann
Aug 1, 1995·Journal of Leukocyte Biology·E MartinR I Lehrer
Sep 1, 1994·Protein Science : a Publication of the Protein Society·W C WimleyS H White
Jun 1, 1994·Research in Microbiology·E Garcia VéscoviE A Groisman
Feb 1, 1995·The Journal of Clinical Investigation·S S HarwigR I Lehrer
Mar 17, 1995·Science·B S SchonwetterM A Zasloff
Aug 1, 1994·Current Opinion in Immunology·T Ganz, R I Lehrer
Dec 1, 1994·Infection and Immunity·S B AleyF D Gillin
Jul 1, 1993·Journal of Bacteriology·I Behlau, S I Miller
Oct 1, 1993·The Journal of Clinical Investigation·E P MolmentiD C Rubin
Jan 1, 1993·Annual Review of Immunology·R I LehrerT Ganz
Oct 1, 1995·Immunology and Cell Biology·R ClancyA Cripps
Mar 1, 1996·Infection and Immunity·B YasinE A Wagar
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·G DiamondC L Bevins
Jun 1, 1997·Infection and Immunity·E M PorterT Ganz

❮ Previous
Next ❯

Citations

Mar 22, 2000·The Journal of Cell Biology·Y S López-BoadoW C Parks
Apr 21, 2004·Journal of Pediatric Gastroenterology and Nutrition·Qian Yuan, W Allan Walker
Apr 12, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Josef Johann SchneiderHans Christian Korting
Jul 26, 2005·DNA Sequence : the Journal of DNA Sequencing and Mapping·Dhanjit Kumar DasAshok Kumar
Apr 23, 2005·Infection and Immunity·Yongming SangTonatiuh Melgarejo
Jul 23, 2005·Scandinavian Journal of Gastroenterology·Karlheinz KiehneKarl-Heinz Herzig
Jun 15, 2007·Inflammatory Bowel Diseases·Jishu Shi
Nov 27, 2009·The Journal of General and Applied Microbiology·Kung-Hao HsuBor-Rung Ou
Mar 5, 2011·Pediatric Research·Patrycja J PuimanIngrid B Renes
Oct 14, 2011·Surgical Endoscopy·Konstantinos E TsimogiannisMichael Pitiakoudis
Feb 23, 2012·Applied Biochemistry and Biotechnology·Nava ChapnikOren Froy
Nov 15, 2013·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·J Michael KlatteMary Anne Jackson
Oct 30, 2014·Mucosal Immunology·B O SchroederJ Wehkamp
Feb 26, 2016·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Haitham A YacoubAhmed M Elazzazy
Nov 15, 2016·Critical Reviews in Biochemistry and Molecular Biology·Phoom Chairatana, Elizabeth M Nolan
Oct 21, 2016·Nature Immunology·Yoshiyuki GotoHiroshi Kiyono
Apr 18, 2000·Infection and Immunity·S M TravisB F Tack
Nov 24, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A WeinbergB A Dale
Nov 25, 2000·Microbiology and Immunology·T TomitaY Ouchi
Jan 15, 2004·American Journal of Respiratory Medicine : Drugs, Devices, and Other Interventions·Alexander M Cole, Alan J Waring
Dec 13, 2000·The Journal of Biological Chemistry·C H ParkT Ganz
Apr 18, 2002·FEMS Microbiology Letters·Nahed IsmailDavid H Walker
Sep 11, 2008·The Journal of Biological Chemistry·Aisling S DuganWalter J Atwood
Apr 4, 2008·Molecular Medicine·Lars SteinstraesserTobias Hirsch
Jan 1, 2008·Interdisciplinary Perspectives on Infectious Diseases·C V Srikanth, Beth A McCormick
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·J W LillardJ R McGhee
Jun 15, 1999·Pediatric Research·K M Huttner, C L Bevins
Apr 7, 2007·Pediatric Research·Ylva Kai-LarsenBirgitta Agerberth
Feb 8, 2000·The Journal of Biological Chemistry·Q YuJ P Tam
Mar 15, 2001·Nature Immunology·T Ganz
Mar 27, 2010·Letters in Applied Microbiology·Y JiangM Li
Oct 27, 2009·Nature Immunology·Nita H SalzmanNicolaas A Bos
Mar 6, 2009·European Journal of Gastroenterology & Hepatology·Jayantha ArnoldMark Busbridge
May 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna Di NardoRichard L Gallo
Nov 3, 2005·Nature Clinical Practice. Gastroenterology & Hepatology·Jan WehkampEduard F Stange

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.