Broad-spectrum enzyme-linked immunosorbent assay for detection of Legionella soluble antigens.

Journal of Clinical Microbiology
P W Tang, S Toma

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed which detected soluble antigens from culture extracts of Legionella pneumophila serogroups 1 to 8, L. micdadei, L. bozemanii serogroups 1 and 2, L. dumoffii, L. gormanii, L. longbeachae serogroups 1 and 2, L. wadsworthii, L. oakridgensis, L. anisa, L. feeleii serogroup 1, and L. jordanis. The assay was approximately 10-fold more sensitive for the eight L. pneumophila serogroups than for the other Legionella species tested. The ELISA detected Legionella antigens in the urine specimens of 25 of 35 patients with L. pneumophila serogroup 1, 3, 4, 6, and 8; L. micdadei; and L. longbeachae serogroup 1 infections. None of the 334 urine specimens from patients with either non-Legionella pneumonia or urinary tract infections was positive. For 10 patients from whom sequential urine specimens were available, Legionella antigens were not detectable from 7 to 19 days after laboratory diagnosis. Test sensitivity was not affected by heavy bacterial contamination. This ELISA offers the detection of a broad spectrum of Legionella antigens by a single test.

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Citations

Dec 3, 2013·World Journal of Microbiology & Biotechnology·Abolfazl GholipourSeyyed Ali Mard
Jan 9, 2015·Clinical Microbiology Reviews·Jeffrey W Mercante, Jonas M Winchell
Feb 1, 1987·European Journal of Clinical Microbiology·P H Edelstein
May 14, 1999·Journal of the American Geriatrics Society·M LoebA McGeer
Dec 16, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J W Den Boer, E P F Yzerman
Jul 5, 2002·Clinical Microbiology Reviews·Barry S FieldsRichard E Besser
May 17, 2001·Journal of Clinical Microbiology·J Horn
Jun 6, 2021·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Akihiro NakamuraUNKNOWN Study of Bacterial Resistance in the Kinki region of japan (SBRK) group
Oct 1, 1991·Chest·V FalcóJ M Martínez Vázquez

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