PMID: 8971020Dec 1, 1996Paper

Brome mosaic virus helicase- and polymerase-like proteins colocalize on the endoplasmic reticulum at sites of viral RNA synthesis

Journal of Virology
M Restrepo-Hartwig, P Ahlquist

Abstract

The helicase-like 1a and polymerase-like 2a proteins of brome mosaic virus (BMV) are required for viral RNA replication in vivo, are present in membrane-bound viral RNA polymerase extracts, and share conservation with the many other members of the alphavirus-like superfamily. To better understand BMV RNA replication and BMV-host interactions, we used confocal microscopy and double-label immunofluorescence to determine and compare the sites of 1a, 2a, and nascent viral RNA accumulation in BMV-infected barley protoplasts. 1a and 2a showed nearly complete colocalization throughout infection, accumulating in defined cytoplasmic spots usually adjacent to or surrounding the nucleus. These spots grew throughout infection and by 16 h postinoculation often assumed a vesicle-like appearance. The BMV RNA replication complex incorporated 5-bromouridine 5'-triphosphate into RNA in vitro and in vivo, allowing immunofluorescent detection of nascent RNA. The cytoplasmic sites of BMV-specific RNA synthesis coincided with the sites of 1a and 2a accumulation, and at the resolution of confocal microscopy, all sites of 1a and 2a accumulation were sites of BMV RNA synthesis. Double-label immunofluorescence detection of selected subcellular markers a...Continue Reading

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Citations

Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A KrolP Ahlquist
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·J DíezP Ahlquist
Mar 22, 2002·Journal of Virology·Rainer GosertSusan C Baker
Feb 14, 2003·Journal of Virology·Yuriko TomitaMasayuki Ishikawa
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·S AsurmendiR N Beachy
Oct 18, 2005·The Journal of General Virology·Min Sook HwangYoung In Park
Jan 1, 2003·Molecular Plant Pathology·John F Bol
Mar 1, 2000·Molecular Plant Pathology·C C Kao, K Sivakumaran
Jan 23, 2016·Journal of Virology·James R ShortAnette Schneemann
Jan 1, 2019·Journal of Plant Biology = Singmul Hakhoe Chi·Pedro Filho Noronha Souza, Fabricio Eulálio Leite Carvalho
Mar 27, 2001·Journal of Virology·P KujalaL Kääriäinen
Jul 15, 2003·Journal of Virology·Paul AhlquistBilly T Dye
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·T YamanakaM Ishikawa
Mar 26, 2003·Annual Review of Phytopathology·Amine O Noueiry, Paul Ahlquist
Jul 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael SchwartzPaul Ahlquist
May 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tadas PanavasPeter D Nagy
Feb 16, 2007·Journal of Virology·Priscilla M Van WynsberghePaul Ahlquist
Jul 24, 2014·Viruses·Inés Romero-Brey, Ralf Bartenschlager
Aug 6, 2014·Archives of Virology·Muhammad AliMuhammad Tahir
May 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Christian BerenWilliam M Gelbart

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