Bromelain Kinetics and Mechanism on Myofibril from Golden Pomfret (Trachinotus blochii)

Journal of Food Science
Xiao FengHongshun Yang

Abstract

Bromelain was used to tenderize golden pomfrets (Trachinotus blochii). The enzyme kinetic model was x=2.447×ln[1+(1332.21×E0S0-1.74)t], which indicated that the degree of hydrolysis (DH, x) was dependent on hydrolysis time (t), the initial concentration of myofibril (S0 ) and bromelain (E0 ). The relationship between the overall hydrolysis rate (v), S0 , E0 , and t is demonstrated as: v=(16.50(E0S0)-1.33)S0 exp {-2.447ln[1+(1332.21E0S0-1.74)t2]}. Sample of 0.40% E0 /S0 was further used to study the effects of hydrolysis time on the changes of proteins, peptides, free amino acids (FAA), and protein nanostructure. SDS-PAGE result showed that myosin heavy chain was degraded dramatically from 22.88% before treatment to 12.03% after 2 min bromelain treatment. Meanwhile, bromelain did not exhibit activity towards actin, trypomyosin, myosin light chain, and troponin C. A general increase of amino acids indicated the increased DH and the preferential cleavage sites of bromelain in the descending order of lysine, glutamic acid, glycine, ornithine, methionine sulfoxide, and alanine. Atomic force microscope images showed that the strip-like structure of myofibril was considerably degraded by bromelain, and the granulation of protein after...Continue Reading

References

Jun 14, 1985·Science·T J Ahern, A M Klibanov
Sep 27, 2002·Current Opinion in Biotechnology·Ole KirkClaus Crone Fuglsang
Aug 8, 2006·Critical Reviews in Food Science and Nutrition·Christine Delbarre-LadratVéronique Verrez-Bagnis
Aug 5, 1994·Biotechnology and Bioengineering·P Gonzàlez-TelloE M Guadix
Jun 23, 2010·Meat Science·Elisabeth Huff LonerganSteven M Lonergan
Aug 9, 2011·Applied Microbiology and Biotechnology·Anindya BasuSusanna Su Jan Leong
Feb 21, 2013·Journal of the Science of Food and Agriculture·Junzhi BuQianqian Jiang
Jun 27, 2014·Applied Microbiology and Biotechnology·Zatul Iffah Mohd ArshadShow Pau Loke
May 18, 2016·Carbohydrate Polymers·Md Abdur RazzakDonghwa Chung
Apr 30, 2017·Journal of Food Science·Hyun Kyung KimSung Keun Jung
May 30, 2017·Meat Science·Per Ertbjerg, Eero Puolanne

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