PMID: 3768340Sep 9, 1986Paper

Bromoacetophenone as an affinity reagent for human liver aldehyde dehydrogenase

Biochemistry
A D MacKerellR Pietruszko

Abstract

Human liver aldehyde dehydrogenase isozymes E1 and E2 (EC 1.2.1.3) are both completely and irreversibly inactivated by bromoacetophenone (2-bromo-1-phenylethanone). Steady-state kinetics with both acetophenone and chloroacetophenone indicated interaction with the same enzyme form as the aldehyde substrate. Saturation kinetics with chloroacetophenone and bromoacetophenone indicated interaction at a specific site on the enzyme surface and gave a dissociation constant similar to that from steady-state kinetics, suggesting that the same processes were being observed by both methods and that the active site may be involved. Protection against inactivation was afforded by chloral and NAD together. Stoichiometry of inactivation showed the first 2 equiv per tetramer to abolish the majority of catalytic activity; 4 equiv inactivated both isozymes with complete loss of esterase, NAD-stimulated esterase, and dehydrogenase activities. Peptide mapping of enzyme modified with [carbonyl-14C]bromoacetophenone of CNBr digests (E1) and tryptic digests (E1 and E2) showed one peptide to be preferentially labeled. The above results together with the similarity of bromoacetophenone to the substrate benzaldehyde suggest bromoacetophenone may react wi...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·V Chowdhry, F H Westheimer
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L C HsuA Yoshida
Jan 1, 1969·Acta Chemica Scandinavica·T K Li, H Theorell
Apr 2, 1973·Biochemical and Biophysical Research Communications·P WilladsenB Zerner
Jan 1, 1967·Advances in Protein Chemistry·S J Singer
Jan 1, 1969·Annual Review of Biochemistry·B L Vallee, J F Riordan
May 15, 1984·European Journal of Biochemistry·J HempelH Jörnvall
Jan 1, 1982·Alcoholism, Clinical and Experimental Research·J D HempelR Pietruszko
May 7, 1982·Science·R C Vallari, R Pietruszko
Nov 1, 1981·Archives of Biochemistry and Biophysics·R C Vallari, R Pietruszko

❮ Previous
Next ❯

Citations

Feb 25, 1987·Biochimica Et Biophysica Acta·A D MacKerell, R Pietruszko
Aug 30, 1991·Biochimica Et Biophysica Acta·M T RyzlakC P Schaffner
Jan 16, 2002·Journal of Biomolecular Structure & Dynamics·G IzaguirreA MacKerell
Oct 1, 1996·Journal of Protein Chemistry·N MukerjeeR Pietruszko
Aug 1, 1987·British Journal of Addiction·P E Nathan
Nov 15, 1992·Archives of Biochemistry and Biophysics·N Mukerjee, R Pietruszko
Sep 8, 1987·Biochemistry·D P AbriolaR Pietruszko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.