Bromodeoxyuridine-labeled viral particles as a tool for visualization of the immediate-early events of human cytomegalovirus infection

Journal of Virology
Kyle Rosenke, Elizabeth A Fortunato

Abstract

We describe here a simple method for labeling the genome of human cytomegalovirus, a large double-stranded DNA virus, with bromodeoxyuridine (BrdU). The labeled DNA was incorporated into viral particles, which were then collected in cell supernatant. To demonstrate the versatility and effectiveness of this method, labeled virions were used to study the immediate-early events of virus-host cell interaction via indirect immunofluorescence microscopy. It is our hope that this new methodology will prove useful in the study of binding, entry and viral genome deposition in diverse virus systems.

References

Apr 1, 1989·The Journal of General Virology·K S RosenthalK Janiga
May 1, 1968·Journal of Virology·C MorganB Mednis
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Citations

Jan 26, 2012·Chemical Communications : Chem Comm·Li-Li HuangZhi-Ke He
Jul 27, 2007·Journal of Virology·Min Hua Luo, Elizabeth A Fortunato
Sep 17, 2010·PLoS Pathogens·Martin ZydekLüder Wiebusch
Jun 5, 2012·Acta Biochimica Et Biophysica Sinica·Yingliang DuanMin-Hua Luo
Mar 17, 2018·Antiviral Chemistry & Chemotherapy·Koh-Hei YamadaNaoki Inoue
Apr 11, 2006·The Journal of General Virology·Chris M Preston, Mary Jane Nicholl

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