Bromodomain-containing protein 4-independent transcriptional activation by autoimmune regulator (AIRE) and NF-κB

The Journal of Biological Chemistry
Fang HuangB Matija Peterlin

Abstract

Autoimmune regulator (AIRE) and nuclear factor-κB (NF-κB) are transcription factors (TFs) that direct the expression of individual genes and gene clusters. Bromodomain-containing protein 4 (BRD4) is an epigenetic regulator that recognizes and binds to acetylated histones. BRD4 also has been reported to promote interactions between the positive transcription elongation factor b (P-TEFb) and AIRE or P-TEFb and NF-κB subunit p65. Here, we report that AIRE and p65 bind to P-TEFb independently of BRD4. JQ1, a compound that disrupts interactions between BRD4 and acetylated proteins, does not decrease transcriptional activities of AIRE or p65. Moreover, siRNA-mediated inactivation of BRD4 alone or in combination with JQ1 had no effects on AIRE- and NF-κB-targeted genes on plasmids and in chromatin and on interactions between P-TEFb and AIRE or NF-κB. Finally, ChIP experiments revealed that recruitment of P-TEFb to AIRE or p65 to transcription complexes was independent of BRD4. We conclude that direct interactions between AIRE, NF-κB, and P-TEFb result in efficient transcription of their target genes.

References

Mar 9, 2002·The Journal of Biological Chemistry·Xin LinB Matija Peterlin
Jul 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anup DeyKeiko Ozato
Aug 30, 2003·Nature Neuroscience·Mollie K MeffertDavid Baltimore
Apr 1, 2004·Molecular Cell·Kirsteen J CampbellNeil D Perkins
Aug 4, 2006·Molecular Cell·B Matija Peterlin, David H Price
Oct 17, 2007·Molecular and Cellular Biology·Irena OvenB Matija Peterlin
Nov 13, 2007·Biochimica Et Biophysica Acta·Ingrid LiivPärt Peterson
Dec 12, 2007·Molecular and Cellular Biology·Zhuoyu NiJohn T Lis
Apr 17, 2008·The Journal of Clinical Investigation·Maureen A SuMark S Anderson
Nov 15, 2008·Nature Reviews. Immunology·Pärt PetersonAna Rebane
Dec 24, 2008·Molecular and Cellular Biology·Bo HuangLin-Feng Chen
Feb 27, 2009·Current Biology : CB·Shankarling Krishnamurthy, Michael Hampsey
Dec 18, 2009·Nature Immunology·Kajsa Wing, Shimon Sakaguchi
Nov 18, 2010·Journal of Cell Science·Karin M RothgiesserMichael O Hottiger
May 17, 2011·Journal of Medicinal Chemistry·Chun-Wa ChungEdwige Nicodeme
Jul 5, 2011·Nucleic Acids Research·Kristina ŽumerB Matija Peterlin
Aug 20, 2011·Wiley Interdisciplinary Reviews. RNA·B Matija PeterlinDavid H Price
Sep 6, 2011·Cell·Jake E DelmoreConstantine S Mitsiades
Feb 9, 2012·Molecular and Cellular Biology·Kristina ŽumerB Matija Peterlin
Jul 24, 2012·Nucleic Acids Research·Koh FujinagaB Matija Peterlin
Mar 5, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristina ZumerB Matija Peterlin
Apr 30, 2013·Biochimica Et Biophysica Acta·Gil Diamant, Rivka Dikstein
Jun 20, 2014·Molecular Biology of the Cell·Hailian BiHuijian Wu
Sep 30, 2014·Molecular Cell·Jonathan D BrownJorge Plutzky
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hideyuki YoshidaDiane Mathis
Jul 2, 2016·The Journal of Biological Chemistry·Wei ShaoB Matija Peterlin
Jan 31, 2017·Nature Immunology·Kushagra BansalDiane Mathis
Aug 3, 2017·Cell Reports·Christopher C EbmeierDylan J Taatjes

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Citations

Mar 29, 2020·Cancers·Durga Khandekar, Venkataswarup Tiriveedhi
Sep 27, 2018·Journal of Medicinal Chemistry·Anand DivakaranWilliam C K Pomerantz

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