PMID: 19137852Jan 14, 2009Paper

Bromophenol blue binding to mammalian albumins and displacement of albumin-bound bilirubin

Pakistan Journal of Biological Sciences : PJBS
B Boon KimS Tayyab

Abstract

Interaction of bromophenol blue (BPB) with serum albumins from different mammalian species, namely, human (HSA), bovine (BSA), goat (GSA), sheep (SSA), rabbit (RbSA), porcine (PSA) and dog (DSA) was studied using absorption and absorption difference spectroscopy. BPB-albumin complexes showed significant differences in the spectral characteristics, i.e., extent of bathochromic shift and hypochromism relative to the spectral features of free BPB. Absorption difference spectra of these complexes also showed variations in the position of maxima and absorption difference (deltaAbs.) values. Absorption difference spectra of different bilirubin (BR)-albumin complexes showed a significant blue shift accompanied by decrease in deltaAbs. values in presence of BPB which were indicative of the displacement of bound BR from its binding site in BR-albumin complexes. These changes in the difference spectral characteristics of BR-albumin complexes were more marked at higher BPB concentration. However, the extent of these changes was different for different BR-albumin complexes. Taken together, all these results suggest that BPB partially shares BR binding site on albumin and different mammalian albumins show differences in the microenvironment...Continue Reading

References

Feb 1, 1990·International Journal of Biological Macromolecules·S Tayyab, M A Qasim
Jan 1, 1986·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·P C Frandsen, R Brodersen
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Mar 21, 1998·Biochemistry and Molecular Biology International·H RashidS Tayyab
Nov 21, 2007·Journal of Photochemistry and Photobiology. B, Biology·Faizah Mohd FaizulSaad Tayyab

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Citations

Dec 1, 1987·Clinical Biochemistry·A KosakaK Nakane
Oct 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P F WrightN H Stacey
Apr 15, 2004·Placenta·E SikkelF P H A Vandenbussche
Mar 5, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sergey OrlovMarie Urbanová
Oct 24, 2002·Biochemical Pharmacology·Nicole A KratochwilPaul R Gerber
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Michael PeakeRenze Bais
Dec 30, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·L ChuniaudF Trivin
Jun 1, 1982·Clinical Biochemistry·R G Ryall, M J Peake
Nov 24, 1998·Journal of Biochemical and Biophysical Methods·M M KhanS Tayyab
Jan 7, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S Tayyab, M K Ali
Aug 2, 2015·Journal of Photochemistry and Photobiology. B, Biology·Jolanta Sochacka
Jul 27, 2021·ACS Sensors·Kamonchanok PhoonsawatWijitar Dungchai

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