Broncho-alveolar inflammation in COVID-19 patients: a correlation with clinical outcome

BMC Pulmonary Medicine
L. PandolfiF. Meloni

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly reached pandemic proportions. Given that the main target of SARS-CoV-2 are lungs leading to severe pneumonia with hyperactivation of the inflammatory cascade, we conducted a prospective study to assess alveolar inflammatory status in patients with moderate to severe COVID-19. Diagnostic bronchoalveolar lavage (BAL) was performed in 33 adult patients with SARS-CoV-2 infection by real-time PCR on nasopharyngeal swab admitted to the Intensive care unit (ICU) (n = 28) and to the Intermediate Medicine Ward (IMW) (n = 5). We analyze the differential cell count, ultrastructure of cells and Interleukin (IL)6, 8 and 10 levels. ICU patients showed a marked increase in neutrophils (1.24 × 105 ml- 1, 0.85-2.07), lower lymphocyte (0.97 × 105 ml- 1, 0.024-0.34) and macrophages fractions (0.43 × 105 ml- 1, 0.34-1.62) compared to IMW patients (0.095 × 105 ml- 1, 0.05-0.73; 0.47 × 105 ml- 1, 0.28-1.01 and 2.14 × 105 ml- 1, 1.17-3.01, respectively) (p < 0.01). Study of ICU patients BAL by electron transmission microscopy showed viral particles inside mononuclear cells confirmed by immunostaining with anti-viral capsid and spike antibodies. IL6 and IL8 were significantly higher ...Continue Reading

References

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Citations

Feb 20, 2021·Virchows Archiv : an International Journal of Pathology·Hans BösmüllerAlexandar Tzankov
Mar 9, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Michael J Ombrello, Grant S Schulert
Apr 21, 2021·Expert Review of Clinical Immunology·Nicola PotereAldo Bonaventura
May 1, 2021·The Journal of Infectious Diseases·Enrique BernalAlfredo Minguela
Jun 11, 2021·The Journal of Allergy and Clinical Immunology·Younes ZaidLouis Flamand
Jul 3, 2021·Nutrients·Hana M A FakhouryKhaled Alkattan
Jun 29, 2021·Frontiers in Cellular and Infection Microbiology·Peter R KvietysKhaled Al-Kattan
Aug 28, 2021·Biomolecules·Raquel ArroyoPaul S Kingma
Sep 4, 2021·Journal of the American College of Cardiology·Agustín Clemente-MoragónBorja Ibáñez
Oct 8, 2021·Frontiers in Immunology·Behazine CombadièreChristophe Combadière
Sep 28, 2021·New Microbes and New Infections·Z M HollidayA Krvavac
Sep 28, 2021·The Korean Journal of Internal Medicine·Dong-Min KimNam-Hyuk Cho

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Methods Mentioned

BETA
PCR
ELISA
flow cytometry

Software Mentioned

Graphpad Prism
Elements AR
RECOVERY
NIS

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