Bronchoabsorption; a novel bronchoscopic technique to improve biomarker sampling of the airway

Respiratory Research
B R LeakerP J Barnes

Abstract

Current techniques used to obtain lung samples have significant limitations and do not provide reproducible biomarkers of inflammation. We have developed a novel technique that allows multiple sampling methods from the same area (or multiple areas) of the lung under direct bronchoscopic vision. It allows collection of mucosal lining fluid and bronchial brushing from the same site; biopsy samples may also be taken. The novel technique takes the same time as standard procedures and can be conducted safely. Eight healthy smokers aged 40-65 years were included in this study. An absorptive filter paper was applied to the bronchial mucosa under direct vision using standard bronchoscopic techniques. Further samples were obtained from the same site using bronchial brushings. Bronchoalveolar lavage (BAL) was obtained using standard techniques. Chemokine (C-C Motif) Ligand 20 (CCL20), CCL4, CCL5, Chemokine (C-X-C Motif) Ligand 1 (CXCL1), CXCL8, CXCL9, CXCL10, CXCL11, Interleukin 1 beta (IL-1β), IL-6, Vascular endothelial growth factor (VEGF), Matrix metalloproteinase 8 (MMP-8) and MMP-9 were measured in exudate and BAL. mRNA was collected from the bronchial brushings for gene expression analysis. A greater than 10 fold concentration of a...Continue Reading

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Citations

Oct 12, 2019·The European Respiratory Journal·Sarah G ChuIvan O Rosas
Nov 23, 2019·Respiratory Research·Kalina R Atanasova, Leah R Reznikov
Jul 13, 2021·British Journal of Pharmacology·Muhammad Waqas SadiqJens M Hohlfeld

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Methods Mentioned

BETA
bronchoalveolar lavage
biopsies
lavage
biopsy
in vitro transcription
Assay
bronchoalveolar

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