Bronchoalveolar lavage cell gene expression in acute lung rejection: development of a diagnostic classifier

Transplantation
Jagadish PatilMarshall I Hertz

Abstract

Acute lung rejection is a risk factor for chronic rejection, which jeopardizes long-term recipient survival. Presently, acute rejection is diagnosed with the use of transbronchial lung biopsies, which are invasive, expensive, and subject to sampling error. We seek to improve acute rejection diagnostics by identifying genes whose expression in bronchoalveolar lavage (BAL) cells best classifies acute rejection versus no rejection. BAL samples were analyzed from 32 subjects whose concurrent histology showed acute rejection (n=14) or no rejection (n=18). Gene expression was measured with Affymetrix microarrays. Quantitative real-time polymerase chain reaction confirmed the microarray results for selected genes. The nearest shrunken centroid method with 10-fold cross validation defined the classification model. A total of 250 iterations of the algorithm were performed to determine the misclassification error rate and the most influential genes in determining classifiers. The estimated overall misclassification rate was <20%. Seven transcripts were present in every classifier, and 52 transcripts were present in >70% of classifiers; these transcripts are related to T-cell function, cytotoxic CD8 activity, and granulocyte degranulation...Continue Reading

References

Apr 1, 1996·American Journal of Respiratory and Critical Care Medicine·A MagnanM Noirclerc
Apr 8, 1999·The New England Journal of Medicine·S M Arcasoy, R M Kotloff
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert TibshiraniGilbert Chu
Aug 21, 2002·American Journal of Respiratory and Critical Care Medicine·Marc Estenne, Marshall I Hertz
Jul 11, 2003·The New England Journal of Medicine·Minnie SarwalOscar Salvatierra
Sep 6, 2003·American Journal of Respiratory and Critical Care Medicine·Vincent J GiminoMarshall I Hertz
Feb 19, 2004·Nature Reviews. Genetics·UNKNOWN Tumor Analysis Best Practices Working Group
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Mar 24, 2005·Canadian Respiratory Journal : Journal of the Canadian Thoracic Society·Anne StephensonR D Levy
Jun 14, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Murali M ChakinalaElbert P Trulock
Jan 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P F HalloranB Kaplan
Apr 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M C DengM R Mehra
Aug 8, 2006·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Elbert P TrulockUNKNOWN International Society for Heart and Lung Transplantation

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Citations

Apr 23, 2009·American Journal of Respiratory and Critical Care Medicine·Vibha N Lama
Oct 14, 2011·Journal of Translational Medicine·Tara L SpiveyDavide Bedognetti
Jan 10, 2009·Proceedings of the American Thoracic Society·Tereza MartinuScott M Palmer
Mar 21, 2009·Expert Review of Molecular Diagnostics·Jamie Murphy, Stephen A Bustin
Oct 31, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·E Bonaccorsi-RianiA Sánchez-Fueyo
Nov 5, 2013·Transplantation Proceedings·C BerasteguiA Román
Apr 23, 2011·Clinics in Chest Medicine·Tereza MartinuScott M Palmer
Aug 3, 2010·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Robin VosGeert M Verleden
Nov 26, 2008·Clinics in Laboratory Medicine·Valeriya Zarkhin, Minnie M Sarwal
Dec 3, 2013·Xenotransplantation·Elizaveta Fasler-KanPeter Meyer
Feb 12, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J R GreenlandG H Caughey
Apr 27, 2013·Current Opinion in Organ Transplantation·Ying-Han R Hsu, Banu Sis
May 20, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Michael MengelRobert B Colvin
Jul 28, 2020·Journal of Clinical Medicine·Yan Y Sanders
Feb 2, 2021·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Bryan D KraftScott L Shofer
May 25, 2021·Seminars in Respiratory and Critical Care Medicine·Benjamin Renaud-PicardTereza Martinu
Apr 2, 2009·Current Opinion in Organ Transplantation

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