Bronchoalveolar lavage fluid from preterm infants with chorioamnionitis inhibits alveolar epithelial repair.

Respiratory Research
Jasper V BeenJ Freek van Iwaarden

Abstract

Preterm infants are highly susceptible to lung injury. While both chorioamnionitis and antenatal steroids induce lung maturation, chorioamnionitis is also associated with adverse lung development. We investigated the ability of bronchoalveolar lavage fluid (BALF) from ventilated preterm infants to restore alveolar epithelial integrity after injury in vitro, depending on whether or not they were exposed to chorioamnionitis or antenatal steroids. For this purpose, a translational model for alveolar epithelial repair was developed and characterised. BALF was added to mechanically wounded monolayers of A549 cells. Wound closure was quantified over time and compared between preterm infants (gestational age < 32 wks) exposed or not exposed to chorioamnionitis and antenatal steroids (>or= 1 dose). Furthermore, keratinocyte growth factor (KGF) and vascular endothelial growth factor (VEGF) were quantified in BALF, and their ability to induce alveolar epithelial repair was evaluated in the model. On day 0/1, BALF from infants exposed to antenatal steroids significantly increased epithelial repair (40.3 +/- 35.5 vs. -6.3 +/- 75.0% above control/mg protein), while chorioamnionitis decreased wound-healing capacity of BALF (-2.9 +/- 87.1 vs....Continue Reading

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Citations

Aug 21, 2010·Journal of Perinatology : Official Journal of the California Perinatal Association·Hyun Ju LeeJung-Hwan Choi
Oct 12, 2010·Journal of Perinatology : Official Journal of the California Perinatal Association·M GantertB W Kramer
Oct 2, 2012·Experimental Cell Research·Coen H M P WillemsJ Freek van Iwaarden
Oct 14, 2011·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Adele FabianoLuigi Atzori
Feb 3, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yoshio MatsuiMasataka Majima
Dec 23, 2017·American Journal of Respiratory and Critical Care Medicine·Bradley WallaceSteven H Abman
May 11, 2021·Frontiers in Immunology·Maame Efua S Sampah, David J Hackam
Jun 11, 2021·ERJ Open Research·Jessica HillasAnthony Kicic

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Methods Mentioned

BETA
bronchoalveolar
lavage
ELISA
bronchoalveolar lavage

Software Mentioned

Image J

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