PMID: 8939175Nov 1, 1996Paper

Bronchoscopy as a research tool for the study of asthma pathogenesis and effects of antiasthma drugs

The Journal of Allergy and Clinical Immunology
R Djukanović

Abstract

Fiberoptic bronchoscopic methods have greatly improved our understanding of asthma pathogenesis and of the mode of action of established and experimental antiasthma drugs. It is probably most appropriate to study bronchoalveolar lavage (BAL) and bronchial biopsy simultaneously because there are major differences between the airway lumen and tissue compartments: for example, T cells are the most abundant cells in endobronchial biopsy samples but form only 10% to 20% of total BAL cells, and eosinophils and mast cells are more numerous in the airway tissue than in the lumina. Immunostaining is currently the most reliable method for enumerating cells and assessing their activation state with a panel of cell surface and intracellular activation markers. In situ hybridization can be used to study a cell's capacity for cytokine production. Newer techniques allow immunohistochemistry of adjacent cell sections to co-localize staining with different antibodies, showing for example that mast cells contain preformed cytokines IL-4, IL-5, and IL-6 and tumor necrosis factor-alpha A combination of immunohistochemistry and in situ hybridization can colocalize messenger RNA transcription to individual cell types; this approach is useful for T c...Continue Reading

Citations

Jan 15, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Cornelia Blume, Donna E Davies
Sep 11, 2016·Therapeutic Advances in Respiratory Disease·Francesco MenzellaLuigi Zucchi
Nov 3, 2016·Therapeutic Advances in Chronic Disease·Francesco MenzellaLuigi Zucchi
Jul 16, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M GagaS Loukides
Feb 18, 2003·The Journal of Allergy and Clinical Immunology·Donna E DaviesStephen T Holgate

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