Brorphine-Investigation and quantitation of a new potent synthetic opioid in forensic toxicology casework using liquid chromatography-mass spectrometry.

Journal of Forensic Sciences
Alex J KrotulskiBarry K Logan

Abstract

New synthetic opioids continue to appear as novel psychoactive substances (NPS) on illicit drug markets. Isotonitazene emerged in mid-2019, becoming the most prevalent NPS opioid in the United States within a few months. Notification by the Drug Enforcement Administration of its intent to schedule isotonitazene in mid-2020 led to its decline in popularity and replacement with a new NPS opioid: brorphine. Brorphine is a potent synthetic opioid, but little information was previously available regarding its toxicity or involvement in impairment and death. Our laboratory developed an assay for the identification and quantitative confirmation of brorphine using standard addition. Quantitative analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In vitro and in vivo metabolism studies were performed using pooled human liver microsomes and authentic biological specimens, respectively, with analysis by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Brorphine was confirmed in 20 authentic forensic cases, commonly found in combination with fentanyl (100%) and flualprazolam (80%). The average concentration of brorphine in blood was 2.5 ± 3.1 ng/mL (median: 1.1 ng/mL, rang...Continue Reading

References

Jan 10, 2017·Drug and Alcohol Dependence·Joji Suzuki, Saria El-Haddad
Jul 6, 2017·Current Molecular Pharmacology·Ludovic VenturaRicardo Jorge Dinis-Oliveira
Oct 5, 2017·Journal of Analytical Toxicology·Donna PapsunBarry K Logan
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Jan 4, 2019·MMWR. Morbidity and Mortality Weekly Report·Lawrence SchollGrant Baldwin
Feb 25, 2020·Journal of Analytical Toxicology·Alex J KrotulskiBarry K Logan
Mar 20, 2020·MMWR. Morbidity and Mortality Weekly Report·Nana WilsonNicole L Davis
Jun 17, 2020·Journal of Analytical Toxicology·Donna M PapsunBarry K Logan
Aug 1, 2020·Archives of Toxicology·Marthe M VandeputteChristophe P Stove
Aug 4, 2020·Journal of Analytical Toxicology·Nick VerougstraeteChristophe Stove
Dec 17, 2020·Journal of Analytical Toxicology·Alex J KrotulskiBarry K Logan

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Citations

Jul 3, 2021·Pharmaceuticals·Pietro BrunettiFrancesco Paolo Busardò
Jul 8, 2021·Journal of Analytical Toxicology·Marthe M VandeputteChristophe P Stove

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