PMID: 2110570May 1, 1990Paper

Brownian motion of inert tracer macromolecules in polymerized and spontaneously bundled mixtures of actin and filamin

The Journal of Cell Biology
L HouF Lanni

Abstract

By use of light microscopy and fluorescence photobleaching recovery, we have studied (a) structures that form in a system composed of copolymerized rabbit muscle actin and chicken gizzard filamin and (b) the Brownian motion of inert tracer macromolecules in this matrix. We have used as tracers size-fractionated fluorescein-labeled ficoll and submicron polystyrene latex particles. In F-actin solutions, the relative diffusion coefficient of the tracer was a decreasing function of both tracer size and actin concentration. Also, a percolation transition for latex particle mobility was found to follow a form suggested by Ogston (Ogston, A. G. 1958. Trans. Faraday Soc. 54:1754-1757) for random filament matrices. The inclusion of filamin before polymerization resulted in increased tracer mobility. Below a filamin dimer-to-actin monomer ratio of 1:140, no structural features were observed in the light microscope. At or above this ratio for all actin concentrations tested, a three-dimensional network of filament bundles was clearly discriminated. Latex particles were always excluded from the bundles. By use of a dialysis optical cell in which polymerization could be initiated with very little hydrodynamic stress, we found that filamin c...Continue Reading

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Citations

Sep 3, 2008·Die Naturwissenschaften·Heinz Penzlin
Dec 30, 2009·The Journal of Physical Chemistry. B·Hugo Sanabria, M Neal Waxham
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Nov 18, 1996·Physical Review Letters·F AmblardS Leibler

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