Brucella abortus vaccine strain RB51 produces low levels of M-like O-antigen

Vaccine
A CloeckaertLaurence A Guilloteau

Abstract

Brucella abortus RB51 is a rough (R) stable vaccine strain used in cattle and is believed to be devoid of O-side chain. We analyzed by use of a panel of monoclonal antibodies (MAbs) directed against seven previously defined O-polysaccharide (O-PS) epitopes the O-chain expression in strain RB51. Two MAbs specific for the C/Y (A=M) and C (M>A) epitopes showed low bindings in ELISA to strain RB51. O-chain expression was further confirmed by Western blot after SDS-PAGE of strain RB51. In particular, the MAb of C (M>A) specificity, showing preferential binding to M-dominant smooth (S) Brucella strains, revealed in strain RB51 a typical smooth-lipopolysaccharide (S-LPS) pattern which resembled that of M-dominant S-LPS. Thus, the results clearly show that strain RB51 produces low levels of M-like O-antigen.

References

Jul 1, 1991·Veterinary Microbiology·G G SchurigN Sriranganathan
Oct 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S C OlsenM G Stevens

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Citations

Nov 5, 2002·Veterinary Microbiology·Gerhardt G SchurigMichael J Corbel
Dec 23, 2003·Infection and Immunity·Jianwu Pei, Thomas A Ficht
Nov 9, 2010·Vaccine·Carlos Chacón-DíazEdgardo Moreno
Dec 24, 2005·Expert Review of Vaccines·Steven C Olsen, W S Stoffregen
Nov 21, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Steven C OlsenPaola M Boggiatto
Dec 30, 2021·Transboundary and Emerging Diseases·Jose María BlascoIgnacio Moriyón

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