Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum

The Journal of Experimental Medicine
Jean CelliJean-Pierre Gorvel

Abstract

The intracellular pathogen Brucella is the causative agent of brucellosis, a worldwide zoonosis that affects mammals, including humans. Essential to Brucella virulence is its ability to survive and replicate inside host macrophages, yet the underlying mechanisms and the nature of the replicative compartment remain unclear. Here we show in a model of Brucella abortus infection of murine bone marrow-derived macrophages that a fraction of the bacteria that survive an initial macrophage killing proceed to replicate in a compartment segregated from the endocytic pathway. The maturation of the Brucella-containing vacuole involves sustained interactions and fusion with the endoplasmic reticulum (ER), which creates a replicative compartment with ER-like properties. The acquisition of ER membranes by replicating Brucella is independent of ER-Golgi COPI-dependent vesicular transport. A mutant of the VirB type IV secretion system, which is necessary for intracellular survival, was unable to sustain interactions and fuse with the ER, and was killed via eventual fusion with lysosomes. Thus, we demonstrate that live intracellular Brucella evade macrophage killing through VirB-dependent sustained interactions with the ER. Moreover, we assign ...Continue Reading

References

Nov 13, 1999·Nature Cell Biology·S R Pfeffer
May 10, 2000·Trends in Cell Biology·W GuoP Novick
Jun 17, 2000·Infection and Immunity·P C HongThomas A Ficht
Jun 17, 2000·Infection and Immunity·G N ArenasL S Mayorga
Nov 9, 2000·Molecular Microbiology·Pascal LestrateJ-J Letesson
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y A Chen, R H Scheller
Mar 22, 2001·Cellular Microbiology·Diego J ComerciRodolfo A Ugalde
Jul 5, 2001·Cellular Microbiology·R M DelrueJ-J Letesson
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·Leigh A KnodlerB B Finlay
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maria Laura BoschiroliDavid O'Callaghan
Jul 31, 2002·The Journal of Cell Biology·Craig R Roy, Lewis G Tilney
Nov 5, 2002·Veterinary Microbiology·Jean Pierre Gorvel, Edgardo Moreno
Nov 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stephan KöhlerJean-Pierre Liautard
Nov 26, 2002·Nature Cell Biology·Jonathan C Kagan, Craig R Roy

Citations

Oct 16, 2009·Medical Microbiology and Immunology·R Martin RoopDaniel W Martin
Jul 27, 2007·Biogerontology·Kevin P HighNammalwar Sriranganathan
Mar 24, 2004·Current Opinion in Microbiology·Jean Celli, Jean-Pierre Gorvel
Mar 24, 2004·Veterinary Microbiology·Beatriz Arellano-ReynosoJean-Pierre Gorvel
May 3, 2005·Veterinary Microbiology·Michelle Wright ValderasR Martin Roop
Feb 26, 2004·FEMS Microbiology Letters·Rose-May DelrueXavier De Bolle
Dec 20, 2007·Expert Reviews in Molecular Medicine·Emilie FugierJean-Pierre Gorvel
May 29, 2012·Animal Health Research Reviews·R Martin Roop
Feb 17, 2009·Journal of Proteome Research·Julie LamontagneEustache Paramithiotis
May 10, 2005·Nature Immunology·Beatriz Arellano-ReynosoJean-Pierre Gorvel
Feb 24, 2006·Nature Reviews. Immunology·Craig R RoyJean-Pierre Gorvel
Nov 20, 2004·Nature Reviews. Microbiology·Jacques BatutDavid O'Callaghan
Jan 28, 2009·Nature Reviews. Microbiology·Jean Pierre GorvelIgnacio Moriyón
Jun 3, 2005·The New England Journal of Medicine·Georgios PappasEpameinondas Tsianos
Apr 9, 2013·Briefings in Functional Genomics·Marie de Barsy, Gilbert Greub
May 9, 2012·The Journal of Infectious Diseases·Radhika GoenkaCynthia L Baldwin
Jan 11, 2013·The Journal of Infectious Diseases·Suzana Pinto SalcedoJean-Pierre Gorvel
Oct 19, 2004·Genome Research·Amélie DricotXavier De Bolle
May 8, 2013·Antimicrobial Agents and Chemotherapy·Edurne ImbuluzquetaMaría J Blanco-Prieto
Apr 16, 2004·Clinical Microbiology Reviews·Douglas A DrevetsRonald A Greenfield
Oct 17, 2007·Infection and Immunity·Elisabeth BillardAntoine Gross
Apr 15, 2009·Infection and Immunity·Hortensia G RolánRenée M Tsolis
Jan 16, 2013·Infection and Immunity·Mariela G Del GiudiceCecilia Czibener
Apr 11, 2007·Infection and Immunity·Hortensia G Rolán, Renée M Tsolis
Aug 24, 2004·Infection and Immunity·Andreas B den HartighRenée M Tsolis
Sep 14, 2010·Journal of Bacteriology·Carola Martínez-NúñezEsteban Chaves-Olarte

Methods Mentioned

BETA
confocal microscopy

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