Brucella outer membrane protein Omp31 is a haemin-binding protein

Microbes and Infection
M Victoria DelpinoPablo C Baldi

Abstract

The expression of haemin-binding proteins (HBPs) in the outer membrane is one of the strategies used by Gram-negative bacteria to obtain iron from the host. No HBP has been described in Brucella spp. We investigated whether Omp31, an outer membrane protein from Brucella with homology to HBPs from Bartonella quintana, is an HBP. Soluble recombinant Omp31 bound specifically to haemin-agarose, while an unrelated Brucella protein (SurA) did not. A similar experiment showed that native Omp31 found in the Brucella suis membrane fraction also binds to haemin-agarose. Recombinant Omp31 was electrophoresed by SDS-PAGE, transferred to nitrocellulose, and incubated with a haemin solution. Haemin bound to Omp31 and to albumin (positive control) but not to SurA. IPTG-induced recombinant Escherichia coli cells expressing Omp31 on their membrane bound significantly more haemin than uninduced cells or controls carrying a similar plasmid without the omp31 gene, showing that Omp31 also binds haemin in a bacterial membrane environment. Viable Brucella ovis cells bound haemin in solution, and this binding was markedly inhibited by preincubation of cells with antibodies to Omp31 and to an exposed prominent loop of the protein, thus showing that Omp...Continue Reading

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Citations

Jun 15, 2007·Infection and Immunity·Paola Caro-HernándezNieves Vizcaíno
Jan 30, 2008·Veterinary Microbiology·Mohamed N SeleemNammalwar Sriranganathan
Feb 26, 2010·FEMS Microbiology Reviews·Stuart D PerkinsHelen S Atkins
Jan 13, 2021·Archives of Microbiology·María Del Socorro Ruiz-PalmaAraceli Contreras-Rodríguez
Feb 12, 2021·Microbiology and Molecular Biology Reviews : MMBR·R Martin RoopDaniel W Martin

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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.