PMID: 8580914Dec 1, 1995Paper

Brush border membrane aminopeptidase-N in the midgut of the gypsy moth serves as the receptor for the CryIA(c) delta-endotoxin of Bacillus thuringiensis

Insect Biochemistry and Molecular Biology
A P ValaitisD H Dean

Abstract

Aminopeptidase-N (AP-N) was purified from gypsy moth (Lymantria dispar, L.) brush border membrane vesicles (BBMV) proteins by mono-Q chromatography and Superdex-75 gel filtration in the presence of the zwitterionic detergent, CHAPS, using FPLC. The purified AP-N, identified by its enzymatic activity, had an apparent size of 100 kDa, and was identified as the unique Bacillus thuringiensis insecticidal toxin, CryIA(c), binding protein. AP-N clearly displayed strong binding to CryIA(c), exhibiting little or no binding to CryIA(a) or CryIA(b), and showing no binding for the coleopteran-specific toxin, CryIIIA. Protein blots of the BBMV proteins probed with biotin-labeled and 125I-labeled insecticidal proteins revealed that CryIAc binds only to 120 kDa protein which is a slightly larger size in comparison to purified AP-N. Antibodies raised against the gypsy moth AP-N demonstrated that the purified AP-N and the 120 kDa CryIA(c) binding protein of total BBMV proteins are antigenically identical.

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Citations

Feb 11, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G HuaH Ikezawa
May 5, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·N S KumaraswamiH Hori
Feb 24, 2010·The Journal of Biological Chemistry·Iván ArenasIsabel Gómez
Jul 8, 2005·Applied and Environmental Microbiology·Shogo AtsumiRyoichi Sato
Feb 24, 2006·Biochemistry. Biokhimii︠a︡·M A DroninaG G Chestukhina
Jun 6, 1998·Bioscience, Biotechnology, and Biochemistry·Y NagamatsuT Katsumoto
Dec 29, 2009·Journal of Insect Physiology·Chenxi LiuYuyuan Guo
Nov 17, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Purushottam R Lomate, Vandana K Hivrale
Jun 10, 2008·Pest Management Science·Derick GeorgeAngharad Mr Gatehouse

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