Brush cytology for the detection of high-risk HPV infection in oropharyngeal squamous cell carcinoma

Cancer Cytopathology
Martina A BroglieSandro J Stoeckli

Abstract

High-risk human papillomavirus (HR-HPV) infection is associated with improved prognosis and a better response to treatment in patients with oropharyngeal squamous cell carcinoma (OPSCC). Brush cytology is a noninvasive method with which to collect cells from the surface of mucosal lesions. The objective of the current study was to assess the performance of OPSCC brush cytology for the detection of HR-HPV. Liquid-based brush cytology specimens were prospectively collected during panendoscopy from 51 patients with OPSCC. Cell suspensions were analyzed with Papanicolaou staining, polymerase chain reaction-based HPV DNA testing, and p16 immunostaining. HPV testing and p16 staining were also performed on paired OPSCC biopsy or surgical resection specimens. The detection of HR-HPV DNA alone and the combined positivity for HR-HPV DNA and p16 protein in dysplastic squamous cells were used to calculate accuracy, sensitivity, specificity, and positive and negative predictive values for HR-HPV detection using brush cytology samples. Approximately 96% of OPSCC brush cytology samples (49 of 51 samples) were classified as satisfactory for evaluation. Dysplastic squamous cells were found in 88% of samples (43 of 49 samples). HPV DNA testing w...Continue Reading

References

May 1, 1991·Japanese Journal of Cancer Research : Gann·H YoshikawaA Iwamoto
Mar 1, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Aimee R KreimerSilvia Franceschi
Jan 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul M WeinbergerAmanda Psyrri
May 15, 2007·The New England Journal of Medicine·Gypsyamber D'SouzaMaura L Gillison
Aug 8, 2007·International Journal of Cancer. Journal International Du Cancer·Serge J SmeetsRuud H Brakenhoff
Oct 2, 2009·International Journal of Cancer. Journal International Du Cancer·Alain C JungBohdan Wasylyk
Jun 10, 2010·The New England Journal of Medicine·K Kian AngMaura L Gillison
Jul 1, 2010·The American Journal of Surgical Pathology·James S LewisSamir K El-Mofty
Oct 23, 2010·Advances in Anatomic Pathology·Anthony N Snow, Jennifer Laudadio
Feb 9, 2011·Head & Neck Oncology·Jean Lacau St GuilyChristiane Mougin
Sep 7, 2011·Cancer Prevention Research·Mark W Lingen
Oct 5, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andrew G SchacheRichard J Shaw
Oct 5, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anil K ChaturvediMaura L Gillison
Apr 24, 2012·Journal of Voice : Official Journal of the Voice Foundation·Harini NaiduGregory A Grillone
Jun 30, 2012·The American Journal of Surgical Pathology·Richard C JordanMaura L Gillison
Feb 13, 2014·British Journal of Cancer·C Grønhøj LarsenC von Buchwald
Jan 22, 2015·The Journal of Pathology·Ian J Groves, Nicholas Coleman

❮ Previous
Next ❯

Citations

Mar 13, 2017·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Ali QureishiStuart Winter
Feb 1, 2017·Current Opinion in Otolaryngology & Head and Neck Surgery·Lauri JouhiAntti Mäkitie
Jun 18, 2017·Clinical Otolaryngology : Official Journal of ENT-UK ; Official Journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·A QureishiS Winter
Apr 30, 2020·Cancers·Barbara KoflerHerbert Riechelmann
May 8, 2018·Experimental and Therapeutic Medicine·Eduardo Augusto RosaRivadávio Fernandes Batista Amorim
May 23, 2021·Archives of Oral Biology·Narges Ghazi, Maryam Khorasanchi
Aug 31, 2021·Seminars in Radiation Oncology·Kristina R DahlstromErich M Sturgis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.