Abstract
Adequate brushing of oral mucosa is important for accurate human papillomavirus (HPV) detection in potentially malignant (oral leukoplakia [OL], oral lichen planus [OLP]) and malignant (oral squamous cell carcinoma [OSCC]) lesions. Since various factors may limit the adequacy of oral brushing and, consequently, the accuracy of HPV detection, modified sampling procedures should be evaluated for their effect on HPV frequency and/or types detected. To compare the HPV frequency in samples obtained by brushing the lesion site with the frequency in samples obtained by brushing an apparently normal adjacent site. The correlation between HPV frequency and keratinization of the site affected by the lesion, as well as sociodemographic variables (age, sex, smoking and drinking habits), was also examined. HPV DNA was detected in brushing samples from 50 patients with OL, 49 with OLP, and 17 with OSCC. Polymerase chain reaction (PCR) amplification was performed by MY09/MY11 and GP05+/GP06+ primers; the HPV type was identified by DNA sequencing and a reverse hybridization (line probe) assay. Data were analyzed by the Z test, the Fisher's exact test, the chi-square test, odds ratio (OR), and a logistic regression model. HPV DNA was detected i...Continue Reading
References
Jul 1, 1992·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·G LawtonI Frazer
Nov 1, 1992·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·H JalalN J Maitland
Sep 19, 1990·Journal of the National Cancer Institute·R M ResnickM M Manos
May 18, 1989·Nature·S Kwok, R Higuchi
Jan 19, 1980·Lancet·J A LowesG M Rees
May 1, 1994·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·C OstwaldT Löning
Sep 23, 1997·Annals of Human Biology·H WalterO Rickards
Nov 12, 1998·Journal of the National Cancer Institute·S M SchwartzD A Galloway
Nov 30, 1999·The British Journal of Oral & Maxillofacial Surgery·P J ThomsonD R Appleton
May 4, 2000·Journal of the National Cancer Institute·H zur Hausen
May 4, 2000·Journal of the National Cancer Institute·M L GillisonD Sidransky
Jan 3, 2001·Clinics in Dermatology·T A Winning, G C Townsend
Jun 13, 2001·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·C S Miller, B M Johnstone
Oct 31, 2001·Archives of Oral Biology·P J ThomsonD R Appleton
Nov 6, 2001·Journal of the National Cancer Institute. Monographs·C A Squier, M J Kremer
Dec 26, 2001·Cancer·J P KlussmannP G Fuchs
Mar 29, 2002·The Journal of Infectious Diseases·Lucia GiovannelliPietro Ammatuna
Jun 5, 2002·Nature Reviews. Cancer·Harald zur Hausen
Aug 16, 2003·Medical Microbiology and Immunology·Christiane OstwaldMalte Barten
Dec 5, 2003·Journal of the National Cancer Institute·Rolando HerreroUNKNOWN IARC Multicenter Oral Cancer Study Group
Mar 18, 2004·Journal of the National Cancer Institute·Elaine M SmithLubomir P Turek
Aug 7, 2004·Journal of Clinical Microbiology·Lucia GiovannelliPietro Ammatuna
Oct 30, 2004·International Journal of Cancer. Journal International Du Cancer·Priya KoppikarRita Mulherkar
Dec 8, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Giuseppina CampisiMatteo D'Angelo
Citations
Oct 15, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A Salem
Mar 25, 2011·Oral Oncology·Nicoletta TermineGiuseppina Campisi
Feb 19, 2011·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Meir Gorsky, Joel B Epstein
Oct 18, 2015·Brazilian Journal of Otorhinolaryngology·Marise da Penha Costa MarquesNatália Oliveira Cruz
Aug 15, 2009·Cancer Epidemiology·Shih-Wei YangChi-Neu Tsai
Jun 25, 2009·Journal of Medical Virology·Nicoletta TermineGiuseppina Campisi
Jan 21, 2009·Periodontology 2000·Jørgen Slots
Feb 22, 2012·Diagnostic Cytopathology·Elke A JarboeLester J Layfield
Feb 22, 2011·Oral Diseases·M R de AraujoF Merletti
Oct 13, 2006·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Giuseppina CampisiLucia Giovannelli
Sep 27, 2014·International Dental Journal·Chetan A PolSuchitra R Gosavi
Dec 18, 2014·BMC Cancer·Seye AbogunrinIke Iheanacho
Jun 27, 2009·Head & Neck Oncology·Ajay Kumar ChaudharyRavi Mehrotra
Sep 20, 2012·Journal of Virological Methods·Patrizia MorbiniMarco Benazzo
May 11, 2013·Archives of Oral Biology·Márcia Rodrigues PayerasFernanda Gonçalves Salum
Jun 14, 2013·ISRN Dentistry·Pratanporn ArirachakaranYong Poovorawan
Aug 30, 2016·PloS One·Junxian MaJie Liu
Nov 4, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Luca ScapoliFrancesco Carinci
Apr 11, 2014·Journal of Clinical Microbiology·Jason J OngChristopher K Fairley
Jan 17, 2019·PloS One·Sara Gomez-ArmayonesMarisa Mena
Dec 12, 2020·The Journal of Evidence-based Dental Practice·Qianhui ShangHao Xu