Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6, production

The Journal of Immunology : Official Journal of the American Association of Immunologists
Nicole J HorwoodBrian M J Foxwell

Abstract

Bruton's tyrosine kinase (Btk), the gene mutated in the human immunodeficiency X-linked agammaglobulinemia, is activated by LPS and is required for LPS-induced TNF production. In this study, we have investigated the role of Btk both in signaling via another TLR (TLR2) and in the production of other proinflammatory cytokines such as IL-1beta, IL-6, and IL-8. Our data show that in X-linked agammaglobulinemia PBMCs, stimulation with TLR4 (LPS) or TLR2 (N-palmitoyl-S-[2, 3-bis(palmitoyloxy)-(2R)-propyl]-(R)-cysteine) ligands produces significantly less TNF and IL-1beta than in normal controls. In contrast, a lack of Btk has no impact on the production of IL-6, IL-8, or the anti-inflammatory cytokine, IL-10. Our previous data suggested that Btk lies within a p38-dependent pathway that stabilizes TNF mRNA. Accordingly, TaqMan quantitative PCR analysis of actinomycin D time courses presented in this work shows that overexpression of Btk is able to stabilize TNF, but not IL-6 mRNA. Furthermore, using the p38 inhibitor SB203580, we show that the TLR4-induced production of TNF, but not IL-6, requires the activity of p38 MAPK. These data provide evidence for a common requirement for Btk in TLR2- and TLR4-mediated induction of two importan...Continue Reading

References

Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·B FoxwellM Feldmann
Aug 30, 2000·Nature·A Aderem, R J Ulevitch
Oct 19, 2001·Biochemical and Biophysical Research Communications·E A GrzybowskaJ A Siedlecki
Feb 22, 2002·European Journal of Biochemistry·Yukihito KabuyamaYoshimi Homma
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sangita MukhopadhyayBalachandran Ravindran
Jan 16, 2003·Immunology Letters·Shizuo Akira, Hiroaki Hemmi
Apr 8, 2003·Nature Immunology·Marina KaraghiosoffMathias Müller
Apr 30, 2003·The Journal of Experimental Medicine·Gordon D BrownSiamon Gordon
Jun 18, 2003·The Journal of Experimental Medicine·Nicole J HorwoodBrian M J Foxwell
Nov 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Akihiro KimuraTadamitsu Kishimoto

❮ Previous
Next ❯

Citations

Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nathan W SchmidtMark H Kaplan
Feb 17, 2007·The Journal of Biological Chemistry·Alla V TsytsykovaAnne E Goldfeld
Jan 18, 2007·The Journal of Biological Chemistry·Takeshi IntoKenji Matsushita
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Young Jun KangJiahuai Han
Feb 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Noha SemaanJean Sibilia
Oct 1, 2008·Journal of Cancer Research and Clinical Oncology·Yigang QianShusen Zheng
Mar 24, 2009·Annual Review of Immunology·Mary Ellen ConleyDario Campana
Sep 18, 2009·The Journal of Biological Chemistry·Caglar CekicThomas C Mitchell
Jan 29, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fui G GohKim S Midwood
Jun 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vijith VijayanStephan Immenschuh
Dec 2, 2011·Annals of the New York Academy of Sciences·Andrea CeruttiKang Chen
Nov 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ying TanAdam Lerner
Oct 9, 2014·Oncotarget·Lyubov ZaitsevaStuart A Rushworth
Dec 17, 2014·Journal of Neuroinflammation·Sabrina CoquenlorgeMichel Neunlist
May 31, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giacomo TampellaRichard G James
Aug 22, 2015·Scientific Reports·Genevra PillingerStuart A Rushworth
Feb 1, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Daisuke AkiAkihiko Yoshimura
Jan 13, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kristen L W WaltonR Balfour Sartor
Jul 3, 2009·Inflammatory Bowel Diseases·Elisabet CantóSilvia Vidal
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martin MelcherWilfried Ellmeier
Mar 3, 2011·The FEBS Journal·Wilfried EllmeierAlexandra Schebesta
Jan 5, 2011·Apoptosis : an International Journal on Programmed Cell Death·Anupriya KhareVineeta Bal
Aug 18, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Theresa H PageMarc Feldmann
May 25, 2011·Cell Research·Joan Ní Gabhann, Caroline A Jefferies
Oct 13, 2007·The Journal of Biological Chemistry·Sarah L DoyleLuke A J O'Neill
Nov 30, 2010·Nature Chemical Biology·Julie A Di PaoloKevin S Currie
Mar 5, 2011·Journal of Leukocyte Biology·Xianghong ZhangMatthew R Rosengart
Jan 1, 2014·European Journal of Immunology·Jingming WangFranck J Barrat
Apr 19, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer C ByrneCaroline A Jefferies
Jan 23, 2015·Frontiers in Immunology·Matthew C MorrisLiwu Li
Mar 4, 2015·Glia·Christiane MenzfeldUwe-Karsten Hanisch
Jun 23, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Benjawan DunkhunthodGriangsak Eumkeb
Jun 17, 2008·Expert Opinion on Therapeutic Targets·Laura L RokoszMaria L Webb

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Hiromichi TaneichiT Miyawaki
The Journal of Immunology : Official Journal of the American Association of Immunologists
Nathan W SchmidtMark H Kaplan
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
Xiaomin ZhangXiaoqing Shi
© 2021 Meta ULC. All rights reserved