Bruton's tyrosine kinase potentiates ALK signaling and serves as a potential therapeutic target of neuroblastoma

Oncogene
Tianfeng LiHui Zhao

Abstract

Aberrant activation of anaplastic lymphoma kinase (ALK) can cause sporadic and familial neuroblastoma. Using a proteomics approach, we identified Bruton's tyrosine kinase (BTK) as a novel ALK interaction partner, and the physical interaction was confirmed by co-immunoprecipitation. BTK is expressed in neuroblastoma cell lines and tumor tissues. Its high expression correlates with poor relapse-free survival probability of neuroblastoma patients. Mechanistically, we demonstrated that BTK potentiates ALK-mediated signaling in neuroblastoma, and increases ALK stability by reducing ALK ubiquitination. Both ALKWT and ALKF1174L can induce BTK phosphorylation and higher capacity of ALKF1174L is observed. Furthermore, the BTK inhibitor ibrutinib can effectively inhibit the growth of neuroblastoma xenograft in nude mice, and the combination of ibrutinib and the ALK inhibitor crizotinib further enhances the inhibition. Our study provides strong rationale for clinical trial of ALK-positive neuroblastoma using ibrutinib or the combination of ibrutinib and ALK inhibitors.

References

Jul 1, 2004·Journal of Cell Science·Akira MotegiTadashi Yamamoto
Feb 2, 2010·Hematology/oncology Clinics of North America·Julie R ParkHuib Caron
Jun 19, 2010·The New England Journal of Medicine·John M Maris
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lee A HonigbergJoseph J Buggy
Oct 29, 2010·The New England Journal of Medicine·Eunice L KwakA John Iafrate
May 3, 2011·Trends in Biochemical Sciences·Michelle C MendozaJohn Blenis
Mar 13, 2012·Annual Review of Biochemistry·Jason A MacGurnScott D Emr
Mar 28, 2012·International Reviews of Immunology·Joseph J Buggy, Laurence Elias
Jun 1, 2012·Frontiers in Oncology·Fabrizio TabbóUNKNOWN European T-Cell Lymphoma Study Group
May 25, 2013·Nature Reviews. Cancer·Nai-Kong V Cheung, Michael A Dyer
Sep 5, 2014·Cell Death & Disease·W GuoH-J Kung
Nov 12, 2014·Annual Review of Medicine·Chrystal U Louis, Jason M Shohet
Feb 26, 2015·Current Cancer Drug Targets·Nick Ka Ming YauVivian Wai Yan Lui
Mar 11, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sean KhozinRichard Pazdur
Jul 4, 2015·Expert Review of Hematology·Rajshekhar ChakrabortyMorie A Gertz
Aug 16, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·R Angelo de ClaroRichard Pazdur
Jan 14, 2016·Future Oncology·David L Tucker, Simon A Rule
Jan 19, 2016·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Sandeep KodityalSai-Hong Ignatius Ou
Mar 10, 2016·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Cory M VelaLeslie A Andritsos
Nov 3, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Erin LarkinsRichard Pazdur
Oct 21, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Robert Roskoski
Jan 5, 2017·Cold Spring Harbor Molecular Case Studies·Vijaykumar R HollaGeorge R Simon
Mar 14, 2017·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Giulio MetroLucio Crinò

❮ Previous
Next ❯

Citations

Feb 7, 2019·Proteomics. Clinical Applications·Maria FrantziHarald Mischak
May 9, 2019·Physiological Reviews·Julhash U Kazi, Lars Rönnstrand
Jul 10, 2019·Veterinary and Comparative Oncology·Susanne GamperlEmir Hadzijusufovic
Sep 14, 2019·The Journal of Pathology·Marialuisa LavitranoEmanuela Grassilli
Oct 5, 2020·Virchows Archiv : an International Journal of Pathology·Charlotte SyrykhCamille Laurent
Jun 25, 2021·Frontiers in Cell and Developmental Biology·Emanuela GrassilliMarialuisa Lavitrano

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
xenograft
electrophoresis
co-immunoprecipitation
Co-IP
ubiquitination
flow cytometry
xenografts
surface plasmon resonance
transfection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.