Bryostatin-1 enhances the maturation and antigen-presenting ability of murine and human dendritic cells

Cancer Research
Yoonkyung DoMitzi Nagarkatti

Abstract

In this study, we investigated the effect of bryostatin-1 (Bryo-1), an antineoplastic agent, on dendritic cell (DC) maturation, activation, and functions. Murine bone marrow-derived DCs on culture with Bryo-1 alone, Bryo-1 + calcium ionophore (CI), but not CI alone exhibited morphologic changes characteristic of mature DCs and expressed increased levels of CD40, CD80, and CD86. Moreover, Bryo-1 + CI-treated DCs exhibited enhanced antigen-presenting ability to naive and antigen-specific T cells and alloreactive T cells. Bryo-1 + CI-mediated activation of DCs involved protein kinase C (PKC), especially PKC-alpha, -delta, and -iota, and addition of PKC inhibitors impaired their ability to activate T cells. Bryo-1 + CI treatment of DCs did not activate mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase, p38 MAPK, or stress-activated protein kinase/c-Jun NH2-terminal kinase pathways. Finally, treatment of DCs with Bryo-1 alone and Bryo-1 + CI, but not CI alone, induced nuclear translocation of nuclear factor kappaB as studied by confocal microscopy. DCs generated from human peripheral blood monocytes or from human cord blood CD34+ hematopoietic stem cells, when cultured with Bryo-1 + CI, also showed matura...Continue Reading

References

Jan 1, 1991·Fortschritte der Chemie organischer Naturstoffe = Progress in the chemistry of organic natural products. Progrès dans la chimie des substances organiques naturelles·G R Pettit
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W S MayL L Sensenbrenner
Feb 1, 1983·The Journal of Experimental Medicine·R M SteinmanM C Nussenzweig
Oct 1, 1994·The Journal of Experimental Medicine·C CauxJ Banchereau
Jul 1, 1996·The American Journal of Physiology·H W LeeJ Bingham Smith
Mar 2, 1999·The Journal of Experimental Medicine·M CellaA Lanzavecchia
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·G HartmannA M Krieg
Feb 19, 2000·Current Opinion in Immunology·B Beutler
Jan 5, 2001·Expert Opinion on Investigational Drugs·P A Philip, J A Zonder
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R E CurielI Espinoza-Delgado
Apr 3, 2002·Seminars in Cancer Biology·Alain P VicariGiorgio Trinchieri
Aug 21, 2002·Anti-cancer Drugs·A Clamp, G C Jayson
Aug 28, 2002·Natural Product Reports·Karl J HaleMark Frigerio
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·John Y KaoJian-Jun Chen

❮ Previous
Next ❯

Citations

Dec 14, 2011·Clinical & Developmental Immunology·Azim HossainAzizul Haque
Jun 26, 2012·Expert Opinion on Drug Discovery·Darren R Flower
Jul 7, 2012·Cytokine & Growth Factor Reviews·Anna Lisa RemoliMarco Sgarbanti
Aug 5, 2015·Pharmacological Reviews·Sébastien AnguilleZwi N Berneman
Nov 9, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Randy R Brutkiewicz
Sep 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Huifen LiIgor Espinoza-Delgado
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jodie M ChallacombeSteven M Ogbourne

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.