BSA-Seq Discovery and Functional Analysis of Candidate Hessian Fly (Mayetiola destructor ) Avirulence Genes

Frontiers in Plant Science
Lucio Navarro-EscalanteJeffrey J Stuart

Abstract

The Hessian fly (HF, Mayetiola destructor) is a plant-galling parasite of wheat (Triticum spp.). Seven percent of its genome is composed of highly diversified signal-peptide-encoding genes that are transcribed in HF larval salivary glands. These observations suggest that they encode effector proteins that are injected into wheat cells to suppress basal wheat immunity and redirect wheat development towards gall formation. Genetic mapping has determined that mutations in four of these genes are associated with HF larval survival (virulence) on plants carrying four different resistance (R) genes. Here, this line of investigation was pursued further using bulked-segregant analysis combined with whole genome resequencing (BSA-seq). Virulence to wheat R genes H6, Hdic, and H5 was examined. Mutations associated with H6 virulence had been mapped previously. Therefore, we used H6 to test the capacity of BSA-seq to map virulence using a field-derived HF population. This was the first time a non-structured HF population had been used to map HF virulence. Hdic virulence had not been mapped previously. Using a structured laboratory population, BSA-seq associated Hdic virulence with mutations in two candidate effector-encoding genes. Using a...Continue Reading

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Citations

Sep 5, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhikui ChengPeng Wang

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Datasets Mentioned

BETA
PRJNA613640

Methods Mentioned

BETA
genotyping
PCR
fluorescence microscopy
reverse transcription PCR
BSA-seq
Fluorescence
Ion leakage assay

Software Mentioned

SSR Locator
script fisher
FASTX
Perl
Phyre2
mpileup2sync
Perl script fst
Perl scripts
R language
BLASTP

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