Buccal micronucleus cytome assay: results of an intra- and inter-laboratory scoring comparison

Mutagenesis
Claudia BolognesiSiegfried Knasmueller

Abstract

The buccal micronucleus cytome (BMCyt) assay is a minimally invasive approach for measuring DNA damage, cell proliferation, cell differentiation and cell death in exfoliated buccal cells. The main limitation for its use is the lack of knowledge about inter- and intra-laboratory variability in scoring micronuclei and other end points included in the cytome approach. In order to identify the main sources of variability across the BMCyt biomarkers, a scoring exercise was carried out between three experienced laboratories using the same set of slides and an identical set of detailed scoring criteria and associated images for the different end points. Single batches of slides were prepared from pooled samples of four groups of subjects characterised by different frequencies of cell types and micronuclei, namely Down syndrome patients, head and neck cancer patients undergoing radiotherapy and two age- and gender-matched control groups. A good agreement among the laboratories in the identification of normal differentiated cells and of micronuclei was obtained. A 3-fold and 20-fold increase in the frequency of micronucleated cells and micronuclei in differentiated cells of Down syndrome patients and in cancer patients, respectively, co...Continue Reading

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Citations

Feb 28, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·B C Gómez-MedaG M Zúñiga-González
May 29, 2016·Mutation Research. Reviews in Mutation Research·Laetitia Gonzalez, Micheline Kirsch-Volders
Nov 26, 2015·Mutation Research. Reviews in Mutation Research·Claudia BolognesiMarcello Ceppi
Sep 18, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Lyda Espitia-PérezJoão A P Henriques
Oct 6, 2016·Chemosphere·Alexandre Endres MarconSílvia Regina Batistuzzo de Medeiros
Nov 11, 2016·Environmental Geochemistry and Health·Diana Paula Silva LinharesArmindo Dos Santos Rodrigues
Dec 30, 2016·Arhiv za higijenu rada i toksikologiju·Banu AykanatSema Burgaz
Feb 13, 2016·Anais Da Academia Brasileira De Ciências·Tatiane de AquinoAlexandre Rieger
Nov 21, 2017·Environmental Science and Pollution Research International·Marcelino Benvindo-SouzaLia Raquel de Souza Santos
Aug 12, 2020·Carcinogenesis·Tahereh SetayeshSiegfried Knasmüller
Aug 18, 2018·Archives of Toxicology·Nuno Guerreiro Oliveira, Ricardo Jorge Dinis-Oliveira
Jun 30, 2019·Environmental Science and Pollution Research International·Klintean WunnapukTippawan Prapamontol
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Mar 30, 2018·ACS Applied Materials & Interfaces·Sangjin OhJaebeom Lee

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