Buccal swabbing as a noninvasive method to determine bacterial, archaeal, and eukaryotic microbial community structures in the rumen

Applied and Environmental Microbiology
S KittelmannP H Janssen


Analysis of rumen microbial community structure based on small-subunit rRNA marker genes in metagenomic DNA samples provides important insights into the dominant taxa present in the rumen and allows assessment of community differences between individuals or in response to treatments applied to ruminants. However, natural animal-to-animal variation in rumen microbial community composition can limit the power of a study considerably, especially when only subtle differences are expected between treatment groups. Thus, trials with large numbers of animals may be necessary to overcome this variation. Because ruminants pass large amounts of rumen material to their oral cavities when they chew their cud, oral samples may contain good representations of the rumen microbiota and be useful in lieu of rumen samples to study rumen microbial communities. We compared bacterial, archaeal, and eukaryotic community structures in DNAs extracted from buccal swabs to those in DNAs from samples collected directly from the rumen by use of a stomach tube for sheep on four different diets. After bioinformatic depletion of potential oral taxa from libraries of samples collected via buccal swabs, bacterial communities showed significant clustering by di...Continue Reading


Feb 1, 1988·Applied and Environmental Microbiology·N BelayL Daniels
Nov 1, 1970·The American Journal of Clinical Nutrition·M P Bryant
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Apr 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paul W LeppDavid A Relman
Mar 9, 2005·The Journal of General and Applied Microbiology·Chagan Irbis, Kazunari Ushida
Sep 1, 2009·Applied and Environmental Microbiology·M ZhouLe Luo Guan
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Aug 17, 2010·Bioinformatics·Robert C Edgar
Jan 22, 2011·Archaea : an International Microbiological Journal·H-P Horz, G Conrads
Jul 19, 2011·Nature Methods·Dan KnightsScott T Kelley
Dec 14, 2011·Applied and Environmental Microbiology·Emma Hernandez-SanabriaLe Luo Guan
May 5, 2012·Bioinformatics·Elmar PruesseFrank Oliver Glöckner
Aug 18, 2012·Human Molecular Genetics·Kathryn E Kemper, Mike E Goddard
Apr 16, 2013·Applied and Environmental Microbiology·Renee M PetriTim A McAllister
Oct 10, 2013·The British Journal of Nutrition·John P GoopyV Hutton Oddy


Feb 7, 2017·Microbial Biotechnology·Alessandro TancaSergio Uzzau
Mar 20, 2016·Applied and Environmental Microbiology·Laura GlendinningGerry McLachlan
Sep 29, 2018·Animal : an International Journal of Animal Bioscience·Stuart Edward DenmanC S McSweeney
Jul 18, 2019·Animal : an International Journal of Animal Bioscience·C VillotM Silberberg
May 30, 2020·Molecular Biology and Evolution·Jaelle C BrealeyKaterina Guschanski
Feb 15, 2019·Frontiers in Immunology·Kieran G Meade, Cliona O'Farrelly
May 8, 2019·Animal : an International Journal of Animal Bioscience·Robert J GruningerTim A McAllister
Oct 29, 2017·Microbiome·Laura GlendinningGerry McLachlan
Aug 28, 2019·Journal of Animal Science and Biotechnology·Joseph H SkarlupkaGarret Suen

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