Bud4 mediates the cell-type-specific assembly of the axial landmark in budding yeast.

Journal of Cell Science
Pil Jung KangHay-Oak Park

Abstract

Cell polarization occurs along a single axis that is generally determined by a spatial cue. Cells of the budding yeast Saccharomyces cerevisiae select a site for polarized growth in a specific pattern depending on cell type. Haploid a and α cells bud in the axial budding pattern, which depends on a transient marker and requires proteins Bud3, Bud4, Axl1 and Axl2. Here, we report that Bud4 functions as a platform that mediates the ordered assembly of the axial landmark at the division site during M and early G1 phase. Whereas Bud4 associates with Bud3 in all cell types and in the absence of Axl1 or Axl2, Bud4 interacts with Axl1 and Axl2 mainly in haploid cells and only in the presence of all other components of the landmark. Bud4 can bind to GTP or GDP, and a GTP-binding-defective Bud4 fails to interact with Axl1 in vitro. The same bud4 mutation leads to mis-localization of Axl1 and disrupts the axial budding pattern, indicating that GTP binding to Bud4 is important for its role in bud-site selection. We also show the cell-type-specific association of the axial landmark with Bud5, a GDP/GTP exchange factor for Rsr1. Despite their expression in all cell types, Bud4 and Axl2 associate with Bud5 specifically in haploid cells and i...Continue Reading

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Citations

Jan 25, 2013·Journal of Cell Science·Pil Jung KangHay-Oak Park
Jun 16, 2012·Genetics·Erfei Bi, Hay-Oak Park
Jan 29, 2014·FEMS Microbiology Reviews·Mithilesh MishraMohan K Balasubramanian
Dec 21, 2013·FEMS Microbiology Reviews·Sophie G Martin, Robert A Arkowitz
Apr 18, 2016·Cellular and Molecular Life Sciences : CMLS·Maria Angeles Juanes, Simonetta Piatti
Jul 9, 2014·The Journal of Cell Biology·Pil Jung KangHay-Oak Park
Aug 10, 2016·Current Biology : CB·Thomas H CheffingsMohan K Balasubramanian
Oct 28, 2017·Molecular Biology of the Cell·Kristi E MillerHay-Oak Park
Sep 14, 2014·Eukaryotic Cell·Lydia R HeasleyMichael A McMurray
Aug 31, 2014·Eukaryotic Cell·Serendipity Zapanta RinonosAlan Michael Tartakoff
Oct 22, 2015·Annual Review of Microbiology·Anum KhanAmy S Gladfelter
Mar 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Gustavo VasenAlejandro Colman-Lerner
Dec 2, 2020·Cells·Werner Karl-Gustav DaalmanLiedewij Laan

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