Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins

Nature
L OrciJ E Rothman

Abstract

Do the coats on vesicles budded from the Golgi apparatus actually cause the budding, or do they simply coat buds (Fig. 1)? One view (the membrane-mediated budding hypothesis) is that budding is an intrinsic property of Golgi membranes not requiring extrinsic coat proteins. Assembly of coats from dispersed subunits is super-imposed upon the intrinsic budding process and is proposed to convert the tips of tubules into vesicles. The alternative view (the coat-mediated budding hypothesis) is that coat formation provides the essential driving force for budding. The membrane-mediated budding hypothesis was inspired by the microtubule-dependent extension of apparently uncoated, 90-nm-diameter membrane tubules from the Golgi apparatus and other organelles in vivo after treatment with brefeldin A, a drug that inhibits the assembly of coat proteins onto Golgi membranes. This hypothesis predicts that tubules will be extended when coat proteins are unavailable to convert tubule-derived membrane into vesicles. Here we use a cell-free system in which coated vesicles are formed from Golgi cisternae to show that, on the contrary, when budding diminishes as a result of immunodepletion of coat protein pools, tubules are not formed at the expense...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·M G WatersJ E Rothman
Mar 6, 1992·Cell·I Mellman, K Simons
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J G DonaldsonR D Klausner
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Nov 1, 1991·Cell·H R Pelham
Mar 1, 1989·The Journal of Cell Biology·E SmytheG Warren
May 1, 1987·Science·M S MooreR G Anderson
Dec 1, 1986·The Journal of Cell Biology·V J Allan, T E Kreis
Jan 1, 1981·Annual Review of Biochemistry·B M Pearse, M S Bretscher
Jan 1, 1993·The Journal of Cell Biology·E B CluettW J Brown

❮ Previous
Next ❯

Citations

Dec 1, 1995·The Journal of Experimental Zoology·B Govindan, P Novick
Feb 1, 1996·Protein Science : a Publication of the Protein Society·J E Rothman
Jun 29, 1994·Molecular and Cellular Biochemistry·J E Schonhorn, M Wessling-Resnick
Oct 17, 2006·The Journal of Membrane Biology·J BéthuneJ Moelleken
Sep 3, 2013·Histochemistry and Cell Biology·Carmen ValenteDaniela Corda
May 24, 1996·Chemistry and Physics of Lipids·S Cockcroft
Jun 10, 1996·Biochimica Et Biophysica Acta·C Harter, F Wieland
Aug 1, 1995·Current Opinion in Cell Biology·N R Salama, R W Schekman
May 22, 2003·Progress in Biophysics and Molecular Biology·Catherine van VlietPaul A Gleeson
Jan 12, 1997·Journal of the Autonomic Nervous System·X E HouA Dahlström
Oct 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christopher StroupeWilliam Wickner
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L OrciJ E Rothman
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A StamnesJ E Rothman
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Narula, J L Stow
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·B GerichC Harter
Nov 1, 1993·The Journal of Cell Biology·L M TraubS Kornfeld
Dec 1, 1993·The Journal of Cell Biology·G TanigawaJ E Rothman
Dec 1, 1993·The Journal of Cell Biology·O KugeJ E Rothman
Feb 1, 1994·The Journal of Cell Biology·Z ElazarJ E Rothman
Mar 1, 1994·The Journal of Cell Biology·S Hara-KugeJ E Rothman
Feb 1, 1995·The Journal of Cell Biology·J Lippincott-SchwartzG S Bloom
Nov 1, 1996·The Journal of Cell Biology·T RoweW E Balch
Jan 27, 2004·Molecular Biology of the Cell·Allen VolchukLelio Orci
Aug 17, 2011·Cold Spring Harbor Perspectives in Biology·Vincent PopoffFelix Wieland
Apr 29, 2011·Cold Spring Harbor Perspectives in Biology·David K Banfield
Oct 31, 2008·Journal of Virology·Hrefna Kristin JohannsdottirAri Helenius
Jan 1, 1996·Annual Review of Cell and Developmental Biology·I Mellman
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Marcus C S LeeRandy Schekman
Dec 11, 2008·PLoS Biology·Mathias BellerBrian Oliver
May 9, 2000·Biochemical and Biophysical Research Communications·M WeidlerP Rösch

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