Budding of retroviruses utilizing divergent L domains requires nucleocapsid.

Journal of Virology
Nana F BelloFadila Bouamr

Abstract

We recently reported that human immunodeficiency virus type 1 (HIV-1) carrying PTAP and LYPX(n)L L domains ceased budding when the nucleocapsid (NC) domain was mutated, suggesting a role for NC in HIV-1 release. Here we investigated whether NC involvement in virus release is a property specific to HIV-1 or a general requirement of retroviruses. Specifically, we examined a possible role for NC in the budding of retroviruses relying on divergent L domains and structurally homologous NC domains that harbor diverse protein sequences. We found that NC is critical for the release of viruses utilizing the PTAP motif whether it functions within its native Gag in simian immunodeficiency virus cpzGAB2 (SIVcpzGAB2) or SIVsmmE543 or when it is transplanted into the heterologous Gag protein of equine infectious anemia virus (EIAV). In both cases, virus release was severely diminished even though NC mutant Gag proteins retained the ability to assemble spherical particles. Moreover, budding-defective NC mutants, which displayed particles tethered to the plasma membrane, were triggered to release virus when access to the cell endocytic sorting complex required for transport pathway was restored (i.e., in trans expression of Nedd4.2s). We also ...Continue Reading

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Citations

Nov 5, 2013·Veterinary Microbiology·R F CookC J Issel
Aug 17, 2012·Journal of Virology·Paola SetteFadila Bouamr
Feb 26, 2013·Viruses·Svenja WolffAllison Groseth
Sep 17, 2013·Cell Host & Microbe·Jörg Votteler, Wesley I Sundquist
Jul 11, 2014·Frontiers in Microbiology·José O MaldonadoLouis M Mansky
Mar 28, 2020·Journal of Virology·Claudia Del VecchioArianna Calistri
Oct 24, 2020·Viruses·Kevin M RoseFadila Bouamr
Mar 7, 2021·Cells·Arianna CalistriCristina Parolin

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