Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the Elm1 kinase to the bud neck.

PLoS Genetics
Laura MerliniSimonetta Piatti

Abstract

The first step towards cytokinesis in budding yeast is the assembly of a septin ring at the future site of bud emergence. Integrity of this ring is crucial for cytokinesis, proper spindle positioning, and the spindle position checkpoint (SPOC). This checkpoint delays mitotic exit and cytokinesis as long as the anaphase spindle does not properly align with the division axis. SPOC signalling requires the Kin4 protein kinase and the Kin4-regulating Elm1 kinase, which also controls septin dynamics. Here, we show that the two redundant ubiquitin-ligases Dma1 and Dma2 control septin dynamics and the SPOC by promoting the efficient recruitment of Elm1 to the bud neck. Indeed, dma1 dma2 mutant cells show reduced levels of Elm1 at the bud neck and Elm1-dependent activation of Kin4. Artificial recruitment of Elm1 to the bud neck of the same cells is sufficient to re-establish a normal septin ring, proper spindle positioning, and a proficient SPOC response in dma1 dma2 cells. Altogether, our data indicate that septin dynamics and SPOC function are intimately linked and support the idea that integrity of the bud neck is crucial for SPOC signalling.

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Citations

Feb 4, 2014·European Journal of Cell Biology·Katja BackhausJürgen J Heinisch
Jul 17, 2015·Molecular Biology of the Cell·Laura MerliniSimonetta Piatti
Apr 18, 2016·Cellular and Molecular Life Sciences : CMLS·Maria Angeles Juanes, Simonetta Piatti
Feb 28, 2013·Cell Cycle·Richard ChahwanStephen P Jackson
Jan 9, 2020·Biological Chemistry·Marian Farkašovský
Apr 7, 2016·The Biochemical Journal·Elizabeth A MarcusOlga Vagin
Oct 22, 2015·Annual Review of Microbiology·Anum KhanAmy S Gladfelter
May 3, 2021·The Journal of Biological Chemistry·Andrew R YoblinskiRaghuvar Dronamraju

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Methods Mentioned

BETA
FACS
fluorescence recovery after photobleaching
immunoprecipitation
immunoprecipitations

Software Mentioned

Leica FW4000
ImageJ
Softworx
MetaMorph

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