Budesonide, fluticasone propionate, and azithromycin do not modulate the membrane vesicle release by THP-1 macrophages and respiratory pathogens during macrophage infection

Inflammopharmacology
Charlotte VolgersFrank R M Stassen

Abstract

Patients with more severe chronic obstructive pulmonary disease frequently experience exacerbations and it is estimated that up to 50% of these exacerbations are associated with bacterial infections. The mainstay treatment for these infection-related exacerbations constitutes the administration of glucocorticoids, alone or in combination with antibiotics. A recent line of evidence demonstrates that many hormones including the steroid beclomethasone can also directly affect bacterial growth, virulence, and antibiotic resistance. The effect of these regimens on the release of potentially virulent and toxic membrane vesicles (MVs) is at present unclear. In this study, we determined the effect of several pharmacological agents on MVs release by and bacterial growth of common respiratory pathogens. We found that neither the release of MVs nor the bacterial growth was affected by the glucocorticoids budesonide and fluticasone. The macrolide antibiotic azithromycin only inhibited the growth of Moraxella catarrhalis but no effects were observed on bacterial MV release at a concentration that is achieved locally in the epithelial lining on administration. The macrophage pro-inflammatory response to MVs was significantly reduced after tr...Continue Reading

References

Oct 31, 1996·Annals of the New York Academy of Sciences·M Fattal-GermanS Berrih-Aknin
Nov 1, 1996·Antimicrobial Agents and Chemotherapy·K M OlsenG D Campbell
Nov 8, 2001·European Journal of Pharmacology·O CulićM J Parnham
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·John R HurstJadwiga A Wedzicha
Nov 29, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L DragoM R Gismondo
Feb 18, 2006·American Journal of Respiratory and Critical Care Medicine·Alberto PapiSebastian L Johnston
Oct 27, 2006·Primary Care Respiratory Journal : Journal of the General Practice Airways Group·John F O'ReillyLeanne Rice
Feb 16, 2008·Nature Reviews. Immunology·Peter J Barnes
Aug 8, 2008·The European Respiratory Journal·M BarbierS Albertí
Nov 29, 2008·The New England Journal of Medicine·Sanjay Sethi, Timothy F Murphy
Jan 23, 2009·The Cochrane Database of Systematic Reviews·Julia Ae WaltersE Haydn Walters
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Terri N Ellis, Meta J Kuehn
Jun 18, 2011·American Journal of Respiratory and Critical Care Medicine·Mona BafadhelChristopher E Brightling
Nov 29, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R LeclercqG Kahlmeter
Oct 12, 2012·International Journal of Chronic Obstructive Pulmonary Disease·Rachel JenDon D Sin
Apr 30, 2013·Journal of Bacteriology·Ian A Macdonald, Meta J Kuehn
Nov 20, 2013·Current Opinion in Pulmonary Medicine·Guy G Brusselle, Guy Joos
Jan 2, 2014·Scientifica·Primrose Freestone
Jan 22, 2014·Nature Reviews. Immunology·Tracy Hussell, Thomas J Bell
Mar 29, 2014·The Cochrane Database of Systematic Reviews·Kayleigh M KewChristopher J Cates
Apr 29, 2014·Journal of Proteomics·Alfonso Olaya-AbrilManuel J Rodríguez-Ortega
May 2, 2014·American Journal of Respiratory and Critical Care Medicine·MeiLan K HanFernando J Martinez
May 6, 2014·Frontiers in Microbiology·Francesco ImperiPaolo Visca
Oct 4, 2014·Journal of Extracellular Vesicles·Anita N BöingRienk Nieuwland
Dec 22, 2014·BMC Microbiology·Carmen SchwechheimerMeta J Kuehn
May 16, 2015·Nature Reviews. Immunology·Maria Kaparakis-Liaskos, Richard L Ferrero
May 17, 2015·Journal of Extracellular Vesicles·María Yáñez-MóOlivier De Wever
Jul 22, 2015·Journal of Extracellular Vesicles·Richard J LobbAndreas Möller
Sep 17, 2015·Nature Reviews. Microbiology·Carmen Schwechheimer, Meta J Kuehn
Apr 2, 2016·The Journal of Clinical Investigation·Jeffrey S Schorey, Clifford V Harding
Jun 12, 2016·Thorax·Sanooj SoniMasao Takata
Mar 4, 2017·Journal of Structural Biology·Na'ama KoifmanYeshayahu Talmon

❮ Previous
Next ❯

Citations

Dec 29, 2020·Frontiers in Microbiology·Negar Mozaheb, Marie-Paule Mingeot-Leclercq

❮ Previous
Next ❯

Methods Mentioned

BETA
size-exclusion chromatography
FCS
flow cytometry
flow cytometric assay
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

GraphPad Prism
Izon Control Suite
qNano

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here