PMID: 18425357Apr 22, 2008Paper

Bufalin induces growth inhibition, cell cycle arrest and apoptosis in human endometrial and ovarian cancer cells

International Journal of Molecular Medicine
Noriyuki TakaiHisashi Narahara

Abstract

Bufalin is a traditional Chinese medicine and it induces apoptosis in certain human tumor cell lines. We investigated the effect of bufalin on three endometrial cancer cell lines, two ovarian cancer cell lines, and on normal human endometrial epithelial cells. Endometrial and ovarian cancer cells were treated with various concentrations of bufalin, and its effect on cell growth, cell cycle, apoptosis, and related measurements was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that all endometrial and ovarian cancer cell lines were sensitive to the growth-inhibitory effect of bufalin, although normal endometrial epithelial cells were viable after treatment with the same doses of bufalin that induced growth inhibition in endometrial and ovarian cancer cells. Cell cycle analysis indicated that their exposure to bufalin decreased the proportion of cells in the S-phase and increased the proportion in the G0/G1 phases of the cell cycle. Induction of apoptosis was confirmed by annexin V staining of externalized phosphatidylserine and loss of the transmembrane potential of mitochondria. This induction occurred in concert with the altered expression of genes related to cell cycle and apoptosi...Continue Reading

Citations

Nov 13, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Meiying LiJisheng Li
Aug 3, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lin JiangYing-Bin Liu
Feb 1, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Noriyuki TakaiHisashi Narahara
Nov 29, 2008·Biotechnology Letters·Ailiang ChenJing Cheng
Jan 25, 2011·Apoptosis : an International Journal on Programmed Cell Death·Yinhui DongHongbo Hu
Jun 12, 2013·Investigational New Drugs·M SlingerlandH Gelderblom
Jun 28, 2012·Mitochondrion·Claudia CerellaMarc Diederich
Dec 28, 2010·International Immunopharmacology·Fanghua QiWei Tang
Jan 24, 2012·Journal of Ethnopharmacology·Shengpeng WangYitao Wang
Oct 1, 2016·Journal of Ethnopharmacology·Xia LiuJian-Chao Wang
Nov 12, 2016·Journal of Ethnopharmacology·An-Cheng HuangJing-Gung Chung
Sep 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Liang MaZi Liu
Nov 22, 2019·Anti-cancer Agents in Medicinal Chemistry·Qian ZhaoBo Han
Sep 10, 2020·Basic & Clinical Pharmacology & Toxicology·Shan SuQinghua Zhang

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis