Abstract
Bufalin, a digoxin-like active component of the traditional Chinese medicine Chan Su, exhibits potent antitumor activities in many human cancers. Bufalin induces mitochondria-dependent apoptosis in cancer cells, but the detailed molecular mechanisms are largely unknown. hTERT, the catalytic subunit of telomerase, protects against mitochondrial damage by binding to mitochondrial DNA and reducing mitochondrial ROS production. In the present study, we investigated the effects of bufalin on the cell viability, ROS production, DNA damage, and apoptosis of CAPAN-2 human pancreatic and CAL-27 human oral cancer cells. Bufalin reduced CAPAN-2 and CAL-27 cell viability with IC50 values of 159.2 nM and 122.6 nM, respectively. The reduced cell viability was accompanied by increased ROS production, DNA damage, and apoptosis and decreased expression of hTERT. hTERT silencing in CAPAN-2 and CAL-27 cells by siRNA resulted in increased caspase-9/-3 cleavage and DNA damage and decreased cell viability. Collectively, these data suggest that bufalin downregulates hTERT to induce mitochondria-dependent apoptosis in CAPAN-2 and CAL-27 cells. Moreover, bufalin increased the phosphorylation of JNK and p38-MAPK in CAPAN-2 and CAL-27 cells, and blocking...Continue Reading
References
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J W Shay, S Bacchetti
Jun 11, 1998·Phytochemistry·L Krenn, B Kopp
Jul 20, 2002·Methods in Enzymology·Shazib Pervaiz, Marie-Véronique Clément
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Yu-Sheng CongJerry W Shay
Apr 15, 2006·Human Molecular Genetics·Janine Hertzog SantosBennett Van Houten
Jul 31, 2007·World Journal of Gastroenterology : WJG·Ke-Qi HanChang-Quan Ling
Jun 25, 2008·Cell Research·Yusheng Cong, Jerry W Shay
Aug 30, 2008·Cancer Science·Satoru KyoMasaki Inoue
Mar 7, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Judith HaendelerStefanie Dimmeler
Apr 4, 2009·Anti-cancer Drugs·Dan LiYunpeng Liu
Jul 14, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Lei SunXunbin Wei
Nov 13, 2010·Cancer Research·Inthrani R IndranShazib Pervaiz
Aug 15, 2012·Acta Haematologica·Zhitu ZhuYunpeng Liu
Aug 28, 2012·International Journal of Oncology·Shih-Chang TsaiJai-Sing Yang
Nov 7, 2012·International Journal of Oncology·Su Hyun HongYung Hyun Choi
Jan 5, 2013·Mutation Research·Evelyn LamyVolker Mersch-Sundermann
Jan 16, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Pei-Hao YinQi Li
Aug 21, 2013·Environmental Toxicology·Ya-Yin ChenJin-Biou Chang
Nov 1, 2013·Oncology Reports·Wei GuBai Li
Nov 13, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Meiying LiJisheng Li
Feb 27, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dewei Wang, Zhenggang Bi
Mar 4, 2014·Journal of Translational Medicine·Zhou-Ji ZhangWei-Zhong Wu
Apr 9, 2014·Anti-cancer Drugs·Shunchao YanYunpeng Liu
May 23, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Xin TianJun Fang
Sep 5, 2014·International Journal of Nanomedicine·Qiang HuZhi-Hai Peng
Oct 3, 2014·Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·Xian-Biao XieJing-Nan Shen
Dec 17, 2014·International Journal of Nanomedicine·Ying LiSiwang Wang
Jan 6, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Da-Wei DingXun Zhang
Citations
Nov 9, 2016·Innate Immunity·Hsiao-Ting ChenYuh-Lin Wu
Feb 22, 2017·Antioxidants·Marko IvancichNeelu Puri
Sep 24, 2020·Phytotherapy Research : PTR·Yue GaoXianli Meng
Feb 1, 2017·Molecular Medicine Reports·Lei ZhangDa-Yong Jin
Aug 23, 2020·Cancers·Adam EckburgNeelu Puri
Mar 29, 2020·Bioscience Reports·Nam-Yi KimChuHee Lee
May 22, 2021·Medical Oncology·Wenhao LuoTaiping Tai Zhang
Aug 8, 2020·European Journal of Pharmacology·Li-Juan DengDong-Mei Zhang