Bufalin Inhibits HCT116 Colon Cancer Cells and Its Orthotopic Xenograft Tumor in Mice Model through Genes Related to Apoptotic and PTEN/AKT Pathways

Gastroenterology Research and Practice
Jie WangTeng Chen

Abstract

Aims. To investigate the anticolorectal cancer (CRC) effects of Bufalin, a bioactive polyhydroxysteroid from Venenum Bufonis, using HCT116 human CRC cell and an established orthotopic xenograft model in mice, and to explore the mechanisms of action. Material and Methods. Cultured HCT116 cells or BALB/c mice with orthotopic tumor were treated by Bufalin (positive control: 5-FU). Cell proliferation, apoptosis, and cycling were determined by MTT, Annexin V/PI staining, and flow cytometry, respectively. In mice, tumor inhibition rate and animal survival were calculated. The expressions of PTEN/phosphate-PTEN, AKT/phosphate-AKT, Bad, Bcl-xl, Bax, or Caspase-3 in cells and/or tumors were determined by Western blot or immunohistochemical staining. Results. Bufalin significantly inhibited cell proliferation and induced cell apoptosis and cycle arrest in a dose/time-dependent manner. In the animal model, Bufalin treatment resulted in significant inhibition of tumor growth and prolonged survival. In the Bufalin-treated cultured cells and/or xenograft tumors, the expressions of PTEN, Bad, Bax, and Caspase-3 were significantly increased, while p-AKT and Bcl-xL significantly decreased. Conclusions. Our results indicate that Bufalin inhibit ...Continue Reading

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Citations

Mar 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Betty Yuen Kwan LawVincent Kam Wai Wong
Aug 24, 2017·The American Journal of Chinese Medicine·Yo-Han HanSeung-Heon Hong
Nov 27, 2019·World Journal of Stem Cells·Audrey VincentIsabelle Van Seuningen
Apr 21, 2020·World Journal of Gastroenterology : WJG·Florian BürtinMichael Linnebacher
Apr 26, 2018·Molecular Medicine Reports·Haiyong WangZhiqiang Meng
May 8, 2018·Molecular and Clinical Oncology·Jie WangTeng Chen
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dhanasekhar ReddyPreetam Ghosh
Aug 8, 2020·European Journal of Pharmacology·Li-Juan DengDong-Mei Zhang

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Methods Mentioned

BETA
xenograft
scraping
Assay
flow cytometry

Software Mentioned

Pro
Image
SPSS

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