Buffy coat platelets stored in apyrase, aprotinin, and ascorbic acid in a suspended bag: combined strategies for reducing platelet activation during storage

Transfusion
Z R MrowiecE G Puszkin

Abstract

Platelet activation is an important factor impeding the clinical effectiveness of platelet transfusions. In this study, platelet concentrates (PCs) were prepared by a novel suspended-bag buffy coat technique that was followed by the addition of a mixture of platelet activation inhibitors to the storage bag. In vitro platelet function was evaluated in PCs prepared by the suspended-bag buffy coat technique and stored at 22 degrees C for 5 days in the presence of (n = 12) or absence (n = 12) of apyrase, ascorbic acid, and aprotinin (AAA). Platelets from AAA-incubated PCs demonstrated mean ATP levels 17 percent (p < 0.004), 13 percent (p < 0.02), and 22 percent (p < 0.003) higher than those measured in parallel control PCs on Days 1, 3, and 5, respectively. Similarly, on Days 3 and 5 of storage, respectively, 45-percent (p < 0.001) and 50-percent (p < 0.001) greater ADP-induced maximum aggregation was observed in AAA-incubated PCs than was seen in control preparations. AAA-incubated PCs demonstrated alpha-granule membrane protein-140 expression 92 percent (p < 0.01), 133 percent (p < 0.003), and 104 percent (p < 0.001) below that in control PCs on Days 1, 3, and 5, respectively. At similar intervals, a significant increase in recov...Continue Reading

Citations

Jan 30, 2004·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Kenneth Wong, Xuebin Li
Aug 10, 1999·Critical Reviews in Oncology/hematology·L L Horstman, Y S Ahn
Dec 31, 1997·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Z R MrowiecE G Puszkin
Nov 3, 1998·Transfusion Medicine Reviews·H M Rinder, K A Ault
Apr 26, 2006·Transplant Immunology·Shosaku NomuraKunio Hayashi
Jun 12, 2010·Blood·Shaun P Jackson, Simone M Schoenwaelder

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