Buffy coat smear or Knott's test: which to choose for canine microfilaria screening in field studies?

Veterinary Clinical Pathology
Ricardo MarcosP Puente-Payo

Abstract

In recent years, an increasing number of cases of canine dirofilariasis have been reported worldwide. However, the rate of infection in dogs is largely unknown in many remote areas, and the importance of field studies for determination of the prevalence of canine dirofilariasis in such areas is well recognized. The detection of microfilariae by the modified Knott's test (MKT) is a recommended screening method for canine dirofilariasis. The purposes of this study were to compare the diagnostic sensitivity of the MKT with the buffy coat smear method (BCS), and to evaluate the utility of these 2 methods under field study conditions. One hundred and fifty dogs of the Maio Island of Republic of Cabo Verde, were screened for microfilariae using MKT and BCS. The results of the 2 methods were generated in a blinded manner and statistically compared. The detection rate was 4.67% with the MKT and 5.33% with BCS, which is statistically not different. The latter allowed a morphologic identification of Dirofilaria repens (later confirmed by molecular biology methods) and an estimation of parasite load, which varied from 15 to 185 microfilariae/mL. The methods MKT and BCS were comparable in terms of diagnostic sensitivity. However, the BCS w...Continue Reading

References

Oct 1, 1970·Journal of Clinical Pathology·J M Goldsmid
Apr 29, 2009·Veterinary Parasitology·Claudio GenchiGiuseppe Cringoli
Apr 10, 2010·Parasites & Vectors·Brigitte MennTorsten J Naucke
Jul 6, 2012·Clinical Microbiology Reviews·Fernando SimónJose Alberto Montoya-Alonso
Oct 24, 2012·Veterinary Parasitology·Cristina PereiraPablo Payo-Puente
Nov 14, 2012·The Journal of Parasitology·Janice L LiottaDwight D Bowman
Nov 19, 2013·Parasitology Research·Katja SilbermayrHans-Peter Fuehrer
Aug 8, 2015·Veterinary Clinical Pathology·Ricardo MarcosAugusto de Matos

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Citations

Feb 28, 2020·Parasites & Vectors·Carolina R F ChagasGediminas Valkiūnas
Feb 23, 2019·Frontiers in Veterinary Science·Caroline E C GoertzPeter Boveng
Mar 9, 2021·Veterinary Parasitology : Regional Studies and Reports·Sergio Villanueva-SazAntonio Fernández

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