PMID: 9173897Apr 1, 1997Paper

Bufo arenarum egg jelly coat: purification and characterization of two highly glycosylated proteins

The Biochemical Journal
S E ArranzMarcelo O Cabada

Abstract

Egg jelly coats from Bufo arenarum are formed by components secreted along the oviduct. These secretion products overlay the oocytes as they transit along the different oviductal portions. In this study, we have isolated two highly glycosylated proteins of the jelly coat, which are secreted almost all the way along the oviduct. Both glycoproteins [designated as highly glycosylated protein (HGP) and low-molecular-mass highly glycosylated protein (L-HGP)] were purified to homogeneity, from the secretion of the caudal oviduct portion, by CsCl density gradient ultracentrifugation. HGP is a high-molecular-mass protein with mucin-like characteristics: high viscosity, a high content of serine and threonine, about 70% carbohydrate by weight, and a protease-resistant domain. Cleavage of disulphide bridges with reducing agents resulted in the release of a single subunit (300000 Da). L-HGP is also a disulphide-cross-linked protein with lower apparent monomeric molecular mass, in the range 100-120 kDa and containing 50% carbohydrate by weight. HGP contains galactose, fucose, N-acetylgalactosamine and sialic acid, but no mannose, suggesting the presence of O-linked oligosaccharides exclusively. The secretion ratio of HGP increases from ceph...Continue Reading

Citations

Nov 26, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Emma D O'BrienSilvia E Arranz
Mar 17, 2006·Biochemical and Biophysical Research Communications·Gabriela Coux, Marcelo O Cabada
Jun 9, 2009·Zygote : the Biology of Gametes and Early Embryos·Claudia A CrespoSilvia N Fernández
Sep 13, 2000·Molecular Reproduction and Development·M L MartínezM O Cabada
Jun 22, 2000·Molecular Reproduction and Development·S E Arranz, M O Cabada
Apr 3, 2009·Cell and Tissue Research·Sirilug MagerdWattana Weerachatyanukul
Aug 26, 1998·Analytical Biochemistry·S A GradiloneM O Cabada

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