Build Your Bioprocess on a Solid Strain-β-Carotene Production in Recombinant Saccharomyces cerevisiae

Frontiers in Bioengineering and Biotechnology
Javiera LópezEduardo Agosin

Abstract

Robust fermentation performance of microbial cell factories is critical for successful scaling of a biotechnological process. From shake flask cultivations to industrial-scale bioreactors, consistent strain behavior is fundamental to achieve the production targets. To assert the importance of this feature, we evaluated the impact of the yeast strain design and construction method on process scalability -from shake flasks to bench-scale fed-batch fermentations- using two recombinant Saccharomyces cerevisiae strains capable of producing β-carotene; SM14 and βcar1.2 strains. SM14 strain, obtained previously from adaptive evolution experiments, was capable to accumulate up to 21 mg/gDCW of β-carotene in 72 h shake flask cultures; while the βcar1.2, constructed by overexpression of carotenogenic genes, only accumulated 5.8 mg/gDCW of carotene. Surprisingly, fed-batch cultivation of these strains in 1L bioreactors resulted in opposite performances. βcar1.2 strain reached much higher biomass and β-carotene productivities (1.57 g/L/h and 10.9 mg/L/h, respectively) than SM14 strain (0.48 g/L/h and 3.1 mg/L/h, respectively). Final β-carotene titers were 210 and 750 mg/L after 80 h cultivation for SM14 and βcar1.2 strains, respectively. O...Continue Reading

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Citations

Dec 19, 2018·Applied Microbiology and Biotechnology·Cassamo Ussemane MussagyJorge Fernando Brandão Pereira
Oct 27, 2020·Frontiers in Bioengineering and Biotechnology·Javiera LópezEduardo Agosin
Jan 1, 2021·Frontiers in Bioengineering and Biotechnology·Jiwon KimSun-Mi Lee
May 1, 2021·Plants·Antonello PaparellaMwafaq Ibdah
Jul 9, 2021·Journal of Biotechnology·Jennifer Hage-HülsmannThomas Drepper
Aug 3, 2021·Applied Microbiology and Biotechnology·Miho TakemuraNorihiko Misawa

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