Building terpene production platforms in yeast

Biotechnology and Bioengineering
Xun Zhuang, Joe Chappell

Abstract

Plants and microbes commonly make terpenes and terpenoids in small amounts and as complex mixtures, and their chemical synthesis is often costly and inefficient. Hence, there are many efforts to create robust and efficient biological production platforms for this interesting class of molecules. In this study, our effort was directed towards building a yeast production platform using an unbiased genetic selection approach. Yeast strain BY4741 was subjected to EMS mutagenesis, followed by selection for growth in the presence of nystatin, squalestatin, and exogenous cholesterol. This unbiased screen selected for mutant yeast lines having a dispensable mevalonate pathway and containing uncharacterized SUE (sterol uptake enhancement) mutations supporting aerobic uptake of exogenous sterol. These mutants were next screened for high level accumulation of farnesol (FOH), an indicator for high level accumulation of the key intermediate FPP, farnesyl diphosphate. To further improve the FPP pool in these mutants, insertional mutations into the ERG9 gene (coding for squalene synthase) were introduced into those lines capable of accumulating ≥50 mg farnesol/L. This generated another series of lines that accumulated farnesol levels over 70 m...Continue Reading

References

Feb 17, 1994·Nature·K C NicolaouK Paulvannan
Jul 1, 1993·Archives of Biochemistry and Biophysics·D ZhangC D Poulter
Jul 8, 1997·Biochemistry·J R MathisJ Chappell
Jan 6, 2000·Archives of Biochemistry and Biophysics·S OkadaJ Chappell
Nov 6, 2001·Applied Microbiology and Biotechnology·S Jennewein, R Croteau
May 22, 2003·Analytical Biochemistry·Linsheng Song
Jun 5, 2003·Nature Biotechnology·Vincent J J MartinJay D Keasling
Apr 1, 1960·The Journal of Clinical Investigation·M D SIPERSTEIN, M J GUEST
Jan 30, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J A Maertens
Oct 3, 2006·Biotechnology and Bioengineering·Shunji TakahashiJoe Chappell
Aug 21, 2007·Biotechnology and Bioengineering·Mohammad A AsadollahiJens Nielsen
Sep 18, 2007·Malaria Journal·Jean-Marie KindermansMelba Gomes
Jan 1, 1982·Biotechnology and Bioengineering·L W HillenN White
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hikaru SekiToshiya Muranaka
Jan 27, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zih-Rou HuangJia-You Fang
Mar 17, 2009·Chemical Research in Toxicology·John E Casida
Oct 7, 2009·Advanced Drug Delivery Reviews·L Harivardhan Reddy, Patrick Couvreur
Sep 30, 2010·Current Pharmaceutical Biotechnology·Hari Narayan BhilwadeTetsuya Konishi
Oct 12, 2010·Science·Parayil Kumaran AjikumarGregory Stephanopoulos
Mar 11, 2011·Biotechnology and Bioengineering·Marc J C FischerFrancis Karst
Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tom D NiehausJoe Chappell
Nov 30, 2011·Applied Microbiology and Biotechnology·Brett A BoghigianBlaine A Pfeifer
Jan 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Patrick J WestfallChris J Paddon
Oct 23, 2012·Methods in Enzymology·Trinh-Don NguyenDae-Kyun Ro
Dec 20, 2012·Drug Design, Development and Therapy·Jean A Yared, Katherine H R Tkaczuk
Oct 15, 2013·Biotechnology and Bioengineering·Jifeng Yuan, Chi Bun Ching

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Citations

Jan 2, 2016·Current Opinion in Biotechnology·Michael D LeavellChris J Paddon
May 2, 2016·Current Opinion in Biotechnology·Evangelos C Tatsis, Sarah E O'Connor
Jun 18, 2016·Current Opinion in Chemical Biology·Ziga ZebecRainer Breitling
Jun 21, 2016·Biochimica Et Biophysica Acta·Kristin B LinscottJoe Chappell
Apr 22, 2017·Critical Reviews in Biotechnology·Kalaivani Paramasivan, Sarma Mutturi
Feb 6, 2018·Journal of Industrial Microbiology & Biotechnology·Jong Yun HanEui-Sung Choi
Oct 19, 2017·Nucleic Acids Research·Karel MiettinenAlain Goossens
Jul 7, 2018·Plant Biotechnology Journal·Chase KempinskiJoe Chappell
Mar 7, 2019·Organic & Biomolecular Chemistry·Allen R ChackoJon O Freeman
Feb 7, 2020·Microbial Cell Factories·Gina M GeiselmanJohn M Gladden
Feb 23, 2019·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Wissam FarhatPer-Olof Syrén
Apr 11, 2019·Frontiers in Bioengineering and Biotechnology·Nisarg GohilVijai Singh
Mar 20, 2019·Microbial Cell Factories·Xun ZhuangJohn M Gladden
Oct 15, 2020·Scientific Reports·Mizuki TatenoChase F Kempinski
Jan 5, 2021·Applied Microbiology and Biotechnology·Govinda R NavaleSandip S Shinde
Jan 10, 2021·Bioresource Technology·Wen-Yan TangHu-Hu Liu
Apr 9, 2020·Journal of Agricultural and Food Chemistry·So-Hee SonJu Young Lee
Jul 15, 2021·Critical Reviews in Biotechnology·Chun-Li LiuTianwei Tan
Oct 12, 2017·ACS Synthetic Biology·Ammara KhalidShunji Takahashi
Aug 29, 2017·Journal of Agricultural and Food Chemistry·Kalaivani Paramasivan, Sarma Mutturi

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