Bulked segregant RNA-sequencing (BSR-seq) identified a novel rare allele of eIF4E effective against multiple isolates of BaYMV/BaMMV.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Lijie ShiPing Yang

Abstract

A novel rare allele of the barley host factor gene eIF4E for BaMMV/BaYMV infection was identified in an Iranian landrace that showed broad resistance to barley yellow mosaic virus disease, and molecular markers facilitating efficient selection were developed. The soil-borne yellow mosaic virus disease caused by different strains of barley yellow mosaic virus (BaYMV) and barley mild mosaic virus (BaMMV) is a major threat to winter barley (Hordeum vulgare) production in Europe and East Asia. However, the exploration of resistant germplasm or casual genes for barley breeding is rather limited in relation to the rapid diversification of viral strains. Here, we identified an Iranian barley landrace 'HOR3298,' which represented complete resistance to BaYMV and BaMMV. In contrast to rym4 and rym5, which act as the predominant source in Europe and East Asia for breeding resistant cultivars over decades and which have been overcome by several virulent isolates, this landrace showed broad-spectrum resistance to multiple isolates of BaYMV/BaMMV in the fields of Germany and China. By employment of bulked segregant RNA sequencing, test for allelism, and haplotype analysis, a recessive resistance gene in 'HOR3298' was genetically mapped coin...Continue Reading

References

Nov 26, 2002·Trends in Genetics : TIG·Michael M NeffMichael Kalishman
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y OkadaK Ito
Jun 28, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B RugeP Wehling
Jun 9, 2005·The Plant Journal : for Cell and Molecular Biology·Nils SteinAndreas Graner
Jul 14, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B Ruge-WehlingP Wehling
Jan 10, 2008·The Plant Journal : for Cell and Molecular Biology·Carine CharronCarole Caranta
Jul 19, 2008·Archives of Virology·H NishigawaT Natsuaki
Sep 1, 2003·Molecular Plant Pathology·Konstantin KanyukaMichael J Adams
Nov 1, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hiroomi KaiTakahide Baba
May 16, 2012·PloS One·Sanzhen LiuPatrick S Schnable
Mar 5, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Thomas LüpkenFrank Ordon
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Feb 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ping YangNils Stein
May 23, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ping YangNils Stein
Jun 12, 2014·Genome Biology·Martin MascherNils Stein
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Jun 17, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Awais RasheedZhonghu He
Nov 11, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ping YangNils Stein
May 16, 2017·Nature Biotechnology·Anupriya Kaur ThindSimon G Krattinger
Jul 18, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yong WangZhiyong Liu
Jan 4, 2018·BMC Plant Biology·Satyvaldy JatayevYuri Shavrukov
Jun 4, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Mingqin ShaoGuorong Zhang
Aug 1, 2003·Plant Disease·M A AchonC Rubies-Autonell

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Citations

Nov 7, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Wei HuaChengdao Li
Feb 3, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Congcong JiangPing Yang
Dec 3, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hélène PidonNils Stein
Feb 20, 2021·Breeding Science·Kaori OkadaKiyoshi Namai
Jun 3, 2021·International Journal of Molecular Sciences·Antonia MoresDaniela Marone
Jul 3, 2021·Plants·Daniela MaroneGrazia M Borrelli

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
electrophoresis
genotyping

Software Mentioned

HaplotypeCaller
qPCR
MEGA
Sequencher
GATK
dCAPS Finder

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