PMID: 8588736Aug 1, 1995Paper

Buoyant density of Escherichia coli is determined solely by the osmolarity of the culture medium

Archives of Microbiology
W W BaldwinA L Koch

Abstract

In previous studies, we had shown that the buoyant density of Escherichia coli is determined by the osmolarity of the growth medium by varying the osmolarity of the medium with NaCl or sucrose. However, the buoyant density of the cells always exceeded that of the growth medium. Here we determined the effect of medium with a buoyant density greater than the expected buoyant density of cells by adding Nycodenz to Luria broth. Percoll gradients of cells were analyzed by laser light scattering. The buoyant density for 125- and 375-mOsM-grown cells was 0.002 g/ml and 0.003 g/ml more, respectively, for cells grown in the presence of Nycodenz than those grown without Nycodenz, while the buoyant density of 250-mOsM-grown cells was 0.005 g/ml less for cells grown in the presence of Nycodenz than those grown without Nycodenz. Cells grown in 500-mOsM medium with or without Nycodenz had the same buoyant density. The buoyant density of cultures grown in defined medium was the same as those grown in rich medium, with only the medium osmolarity correlating to buoyant density. We conclude from these experiments that neither buoyant density nor chemical make-up of the medium determines the buoyant density of cells grown in that medium. Only the...Continue Reading

Citations

Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrea K BryanScott R Manalis
Jun 6, 2003·Applied and Environmental Microbiology·Tomohiko NishinoKazuhiro Kogure
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Peter R SalamoneThomas W Okita
Mar 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dan DavidiRon Milo
Apr 20, 2012·PloS One·Teuta Pilizota, Joshua W Shaevitz
Nov 9, 2018·MSphere·Raghav VijArturo Casadevall
Jun 11, 2020·International Journal of Molecular Sciences·Nat SakolToshimichi Fujiwara
Jan 1, 2021·Biomicrofluidics·Michael S GerltJürg Dual
Jul 10, 2021·EcoSal Plus·Gwanggyu SunMarkus W Covert

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