PMID: 8946854Dec 1, 1996Paper

Buried polar residues and structural specificity in the GCN4 leucine zipper

Nature Structural Biology
L GonzalezT Alber

Abstract

A conserved asparagine (Asn 16) buried in the interface of the GCN4 leucine zipper selectively favours the parallel, dimeric, coiled-coil structure. To test if other polar residues confer oligomerization specificity, the structural effects of Gln and Lys substitutions for Asn 16 were characterized. Like the wild-type peptide, the Asn 16Lys mutant formed exclusively dimers. In contrast, Gln 16, despite its chemical similarity to Asn, allowed the peptide to form both dimers and trimers. The Gln 16 side chain was accommodated by qualitatively different interactions in the dimer and trimer crystal structures. These findings demonstrate that the structural selectivity of polar residues results not only from the burial of polar atoms, but also depends on the complementarity of the side-chain stereochemistry with the surrounding structural environment.

References

Feb 1, 1991·International Journal of Biological Macromolecules·J F Conway, D A Parry
Oct 1, 1990·International Journal of Biological Macromolecules·J F Conway, D A Parry
Oct 5, 1990·Journal of Molecular Biology·J A IppolitoD W Christianson
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Jul 20, 1973·Science·C B Anfinsen
Aug 1, 1995·Current Opinion in Biotechnology·J R Desjarlais, T M Handel
Apr 1, 1995·Protein Science : a Publication of the Protein Society·K A DillH S Chan
Aug 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Kamtekar, M H Hecht
Mar 28, 1995·Biochemistry·H WendtH R Bosshard
Feb 1, 1994·Protein Science : a Publication of the Protein Society·Z S Hendsch, B Tidor
Sep 1, 1994·Nature·P B HarburyT Alber
Aug 1, 1995·Protein Science : a Publication of the Protein Society·D N Woolfson, T Alber
Aug 1, 1995·Current Opinion in Structural Biology·S F BetzW F DeGrado
Oct 1, 1995·Protein Science : a Publication of the Protein Society·M W West, M H Hecht
Jun 1, 1996·Nature Structural Biology·L GonzalezT Alber
Feb 1, 1996·Current Opinion in Structural Biology·M H CordesR T Sauer

❮ Previous
Next ❯

Citations

May 30, 1998·Protein Science : a Publication of the Protein Society·N LiuR M Thomas
Nov 25, 1998·Protein Science : a Publication of the Protein Society·E G HutchinsonD N Woolfson
Aug 31, 2002·Biophysical Journal·Holly GratkowskiJames D Lear
Jun 11, 2002·Journal of Molecular Biology·Jie LiuMin Lu
Oct 18, 2002·Advanced Drug Delivery Reviews·Y Bruce Yu
Aug 10, 2001·Current Opinion in Structural Biology·M G Oakley, J J Hollenbeck
Jan 23, 1999·Current Opinion in Chemical Biology·G A Lazar, T M Handel
Jul 13, 2005·Biochemistry·Maneesh K YadavM Reza Ghadiri
Dec 8, 2005·Biochemistry·Christina M Taylor, Amy E Keating
Jan 11, 2011·Biochemistry·Helen Y FanG Andrew Woolley
May 15, 2010·Journal of the American Chemical Society·Jay D SteinkrugerSamuel H Gellman
Feb 3, 2012·Journal of the American Chemical Society·Jay D SteinkrugerSamuel H Gellman
Aug 25, 2012·Journal of the American Chemical Society·Aimee L BoyleDerek N Woolfson
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Yingzhen YangThomas Jack
Jan 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erik B HadleySamuel H Gellman
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marcus D HartmannBirte Hernandez Alvarez
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sozanne R SolmazIvo Melcák
Nov 7, 2012·Bioinformatics·Thomas L VincentDerek N Woolfson
Nov 21, 2001·Protein Science : a Publication of the Protein Society·K DuttaS M Pascal
Mar 27, 2001·Protein Science : a Publication of the Protein Society·H ZhuJ C Hu
Mar 27, 2001·Protein Science : a Publication of the Protein Society·A Ababou, J R Desjarlais
Apr 21, 1999·Protein Science : a Publication of the Protein Society·S Nautiyal, T Alber
Jan 13, 2000·Protein Science : a Publication of the Protein Society·G A LazarT M Handel
Jun 13, 2000·Protein Science : a Publication of the Protein Society·C L Boon, A Chakrabartty
Jun 14, 2000·Journal of Virology·J M MatthewsJ P Mackay
Aug 17, 2004·Journal of Virology·Michelle J WestDerek N Woolfson
Jun 29, 2000·Annual Review of Biochemistry·W F DeGradoA Lombardi
Feb 5, 2004·Genome Biology·Jessica H FongMona Singh
Jan 10, 2013·PLoS Computational Biology·Ranjan V MannigeEugene I Shakhnovich
Feb 17, 2011·The Journal of Chemical Physics·Jason Gee, M Scott Shell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.