Burkholderia (basonym Pseudomonas) cepacia binding to lipid receptors.

Infection and Immunity
F A SylvesterJ Forstner

Abstract

Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial cells via a 22-kDa adhesin located on unique cable pili. However, a second receptor, thought to be lipid in nature, was also identified on cells and appeared to serve as the major cell receptor for poorly piliated or nonpiliated isolates. In the present study in vitro approaches were used to identify putative lipid receptors for B. cepacia and to explore the nature of the binding interaction. As judged by thin-layer chromatography overlay assays, the best receptors were digalactosylceramide and globotriosylceramide (Gb(3)). Both contain and unsubstituted terminal Gal alpha 1-4Gal sequence. B cepacia also bound moderately to galactosylceramide, gangliotriosylceramide, and gangliotetraosylceramide. Binding to glycolipids was not affected by tetramethylurea, a hydrophobic-bond-breaking adhesin for GB(3). Binding to glycolipids was not affected by tetramethylurea, a hydrophobic-bond-breaking agent. Binding was influenced by the structure of the ceramide, which probably affects the presentation of the agent. Binding was influenced by the...Continue Reading

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Citations

Dec 17, 2008·Journal of Medical Microbiology·Siobhán McClean, Máire Callaghan
Mar 18, 2000·Infection and Immunity·U S SajjanJ F Forstner
May 9, 2002·British Medical Bulletin·C Anthony Hart, Craig Winstanley
Mar 19, 2002·Cellular Microbiology·Umadevi SajjanJanet Forstner
Aug 18, 2004·Journal of Medical Microbiology·Richard Thomas, Tim Brooks
Feb 18, 2011·Bioengineered Bugs·Richard J Thomas
May 23, 2001·Microbes and Infection·C D MohrC A Herfst

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