Burkholderia pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh

PLoS Neglected Tropical Diseases
Md Shariful Alam JilaniJalaluddin Ashraful Haq

Abstract

Melioidosis, caused by Burkholderia pseudomallei, is an endemic disease in Bangladesh. No systematic study has yet been done to detect the environmental source of the organism and its true extent in Bangladesh. The present study attempted to isolate B. pseudomallei in soil samples and to determine its seroprevalence in several districts in Bangladesh. Soil samples were collected from rural areas of four districts of Bangladesh from where culture confirmed melioidosis cases were detected earlier. Multiple soil samples, collected from 5-7 sampling points of 3-5 sites of each district, were cultured in Ashdown selective media. Suspected colonies of B. pseudomallei were identified by biochemical and serological test, and by polymerase chain reaction (PCR) using 16s rRNA specific primers. Blood samples were collected from 940 healthy individuals of four districts to determine anti- B. pseudomallei IgG antibody levels by indirect enzyme linked immunosorbent assay (ELISA) using sonicated crude antigen. Out of 179 soil samples, B. pseudomallei was isolated from two samples of Gazipur district which is located 58 km north of capital Dhaka city. Both the isolates were phenotypically identical, arabinose negative and showed specific 550bp...Continue Reading

References

Jun 1, 1978·Journal of Clinical Pathology·A VollerD E Bidwell
Aug 1, 1991·Postgraduate Medical Journal·C C KibblerS G Spiro
Oct 1, 1990·Journal of Medical Microbiology·V WuthiekanunN J White
May 1, 1989·The Journal of Infectious Diseases·W ChaowagulN Pitakwatchara
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·M J StruelensD A Dance
Jan 29, 1999·Lancet·D A DanceT L Pitt
Oct 27, 1999·Rheumatology·S N HoqueM P Lessing
Feb 16, 2000·Acta Tropica·D A Dance
Feb 16, 2000·Acta Tropica·B J CurrieM J Mayo
May 28, 2003·Lancet·N J White
Apr 18, 1964·Lancet·B G MAEGRAITH, C S LEITHEAD
Apr 16, 2005·Clinical Microbiology Reviews·Allen C Cheng, Bart J Currie
Jul 11, 2006·Journal of Clinical Microbiology·Mongkol VesaratchavestSharon J Peacock
Jun 4, 2010·The American Journal of Tropical Medicine and Hygiene·Direk LimmathurotsakulSharon J Peacock
May 12, 2011·British Medical Bulletin·Direk Limmathurotsakul, Sharon J Peacock
Jul 17, 2012·Transactions of the Royal Society of Tropical Medicine and Hygiene·Rapeephan R MaudeM Abul Faiz
Apr 5, 2013·PLoS Neglected Tropical Diseases·Direk LimmathurotsakulSharon J Peacock

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Citations

Dec 16, 2016·PLoS Neglected Tropical Diseases·Patpong RongkardDirek Limmathurotsakul
Oct 3, 2018·Tropical Medicine and Infectious Disease·Fazle Rabbi ChowdhuryDavid A B Dance
Mar 13, 2020·Clinical Microbiology Reviews·I GassiepR Norton
Apr 15, 2016·PLoS Neglected Tropical Diseases·Kalwaje Eshwara VandanaNalini Sathiakumar
Jul 22, 2017·BMC Research Notes·Subarna DuttaJalaluddin Ashraful Haq
Dec 26, 2018·Wellcome Open Research·David A B DanceDirek Limmathurotsakul
Feb 11, 2021·Saudi Journal of Kidney Diseases and Transplantation : an Official Publication of the Saudi Center for Organ Transplantation, Saudi Arabia·Muhammad Abdur RahimMehruba Alam Ananna

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Methods Mentioned

BETA
PCR
ELISA
electrophoresis

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