Burkholderia pseudomallei RpoS regulates multinucleated giant cell formation and inducible nitric oxide synthase expression in mouse macrophage cell line (RAW 264.7)

Microbial Pathogenesis
P UtaisincharoenS Sirisinha

Abstract

Burkholderia pseudomallei is the causative agent of melioidosis. This bacterium can invade and survive inside the phagocytic and nonphagocytic cells. After internalization, the bacteria can escape from the membrane-bound phagosome into the cytoplasm. Internalised B. pseudomallei can also induce a cell-to-cell fusion, resulting in a multinucleated giant cell (MNGC) formation. In the present study, we demonstrated that B. pseudomallei rpoS null mutant was similar to its wild type parent in its ability to survive and multiply inside the mouse macrophages, but it failed to stimulate MNGC formation. The rpoS mutant was also unable to activate inducible Nitric Oxide Synthase (iNOS) in resting mouse macrophages but in gamma interferon (IFN-gamma)-activated macrophages, the mutant was able to induce significantly higher levels of iNOS and NO when compared with its wild-type counterpart, resulting in a significantly lower number of bacteria inside the infected host cells.

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Citations

Dec 2, 2009·Infection and Immunity·Tao Dong, Herb E Schellhorn
Jan 31, 2009·Infection and Immunity·Andrew GoodyearSteven Dow
Jun 10, 2010·Annual Review of Microbiology·Edouard E GalyovDavid DeShazer
Oct 15, 2014·Expert Review of Anti-infective Therapy·Joshua K StoneMary N Burtnick
Nov 21, 2007·Microbial Pathogenesis·I LengwehasatitP Utaisincharoen
Mar 22, 2013·Biochemical Society Transactions·Leanne M Smith, Robin C May
Apr 24, 2012·Nitric Oxide : Biology and Chemistry·Jessica Jones-CarsonAndrés Vázquez-Torres
Aug 31, 2006·Current Opinion in Infectious Diseases·Sharon J Peacock
Feb 2, 2018·Nature Reviews. Disease Primers·W Joost WiersingaDirek Limmathurotsakul
Mar 13, 2020·Clinical Microbiology Reviews·I GassiepR Norton
Jun 22, 2016·FEMS Microbiology Letters·Sucharat SanongkietSumalee Tungpradabkul
Apr 15, 2009·Journal of Proteome Research·Yupaporn OsiriphunSumalee Tungpradabkul
Dec 1, 2011·EcoSal Plus·Regine Hengge

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